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Secreted class 3 Semaphorins (Semas) trigger cell responses via Plexin-Neuropilin (Nrp) membrane receptor complexes. Here, we report that unconventional Sema3-Nrp preformed complexes are delivered by the CSF from sources including the choroid plexus to Plexin-expressing RGCs via their apical endfeet. Through analysis of mutant mouse models and various ex vivo assays mimicking ventricular delivery to RGCs, we found that two different complexes, Sema3B\/Nrp2 and Sema3F\/Nrp1, exert dual effects on apical endfeet dynamics, nuclei positioning, and RGC progeny. This reveals unexpected balance of CSF-delivered guidance molecules during cortical development.<\/jats:p>","DOI":"10.1126\/sciadv.abo4552","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T18:58:38Z","timestamp":1668797918000},"source":"Crossref","is-referenced-by-count":10,"title":["A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0381-3675","authenticated-orcid":true,"given":"Katrin","family":"Gerstmann","sequence":"first","affiliation":[{"name":"MeLis, CNRS UMR 5284, INSERM U1314, University of Lyon, Universit\u00e9 Claude Bernard Lyon 1, Institut NeuroMyoG\u00e8ne, 8 avenue Rockefeller, 69008 Lyon, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Kindbeiter","sequence":"additional","affiliation":[{"name":"MeLis, CNRS UMR 5284, INSERM U1314, 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