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Increased morphological proximity to synapses occurred in one subpopulation and increased extrasynaptic GLT-1 expression in another. Augmented synapse proximity by astroglia occurred selectively at D2-dopamine receptor\u2013expressing dendrites, while changes in GLT-1 were not neuron subtype specific. mRNA-targeted antisense inhibition of either morphological or GLT-1 plasticity promoted cue-induced heroin seeking. Thus, we show that heroin cues induce two distinct forms of transient plasticity in separate astroglial subpopulations that dampen heroin relapse.<\/jats:p>","DOI":"10.1126\/sciadv.abo7044","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T13:57:31Z","timestamp":1660139851000},"source":"Crossref","is-referenced-by-count":47,"title":["Plasticity in astrocyte subpopulations regulates heroin relapse"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9267-9492","authenticated-orcid":true,"given":"Anna","family":"Kruyer","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3432-5228","authenticated-orcid":true,"given":"Ariana","family":"Angelis","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanza","family":"Garcia-Keller","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6065-5100","authenticated-orcid":true,"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biostatistics &amp; Bioinformatics, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Kalivas","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Medical University of South Carolina, Charleston, SC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2515"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-21-08751.1998"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(00)00067-8"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1871527314666150529144655"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2015.12.022"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopsych.2019.06.026"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.03.040"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.04.032"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100957108"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-061010-113641"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2659-16.2016"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-020-00888-z"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10194"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3100"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b16-01011"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153776"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.1.193"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.1120"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2369-19.2020"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2021.07.004"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-021-01333-5"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1360-0443.1999.9433273.x"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2009.07.016"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/adb.12182"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5129-07.2008"},{"key":"e_1_3_3_27_2","unstructured":"F. 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