{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T04:03:25Z","timestamp":1788840205596,"version":"build-2803163510"},"reference-count":72,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"44","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2022,11,4]]},"abstract":"<jats:p>The generation of a correctly sized cerebral cortex with all-embracing neuronal and glial cell\u2013type diversity critically depends on faithful radial glial progenitor (RGP) cell proliferation\/differentiation programs. Temporal RGP lineage progression is regulated by Polycomb repressive complex 2 (PRC2), and loss of PRC2 activity results in severe neurogenesis defects and microcephaly. How PRC2-dependent gene expression instructs RGP lineage progression is unknown. Here, we use mosaic analysis with double markers (MADM)\u2013based single-cell technology and demonstrate that PRC2 is not cell-autonomously required in neurogenic RGPs but rather acts at the global tissue-wide level. Conversely, cortical astrocyte production and maturation is cell-autonomously controlled by PRC2-dependent transcriptional regulation. We thus reveal highly distinct and sequential PRC2 functions in RGP lineage progression that are dependent on complex interplays between intrinsic and tissue-wide properties. In a broader context, our results imply a critical role for the genetic and cellular niche environment in neural stem cell behavior.<\/jats:p>","DOI":"10.1126\/sciadv.abq1263","type":"journal-article","created":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T13:57:27Z","timestamp":1667397447000},"source":"Crossref","is-referenced-by-count":27,"title":["Tissue-wide genetic and cellular landscape shapes the execution of sequential PRC2 functions in neural stem cell lineage progression"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3183-8207","authenticated-orcid":true,"given":"Nicole","family":"Amberg","sequence":"first","affiliation":[{"name":"Institute of Science and Technology Austria (ISTA), Am Campus 1, 3400 Klosterneuburg, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7462-0048","authenticated-orcid":true,"given":"Florian M.","family":"Pauler","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria (ISTA), Am Campus 1, 3400 Klosterneuburg, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Streicher","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria (ISTA), Am Campus 1, 3400 Klosterneuburg, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2279-1061","authenticated-orcid":true,"given":"Simon","family":"Hippenmeyer","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria (ISTA), Am Campus 1, 3400 Klosterneuburg, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0654-5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12906"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2359-16.2016"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2019.03.004"},{"key":"e_1_3_3_6_2","first-page":"67","article-title":"Proliferative characteristics of the ependymal layer during the early development of the mouse neocortex: A pilot study based on recording the number, location and plane of cleavage of mitotic figures","volume":"116","author":"Smart I. 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