{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T01:32:14Z","timestamp":1792287134320,"version":"4.4.0"},"reference-count":59,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"51","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2022,12,23]]},"abstract":"<jats:p>\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    (Linnaeus, 1762) is the main mosquito vector for dengue and other arboviral infectious diseases. Control of this important vector highly relies on the use of insecticides, especially pyrethroids. The high frequency (&gt;78%) of the L982W substitution was detected at the target site of the pyrethroid insecticide, the voltage-gated sodium channel (Vgsc) of\n                    <jats:italic>A. aegypti<\/jats:italic>\n                    collected from Vietnam and Cambodia. Alleles having concomitant mutations L982W\u00a0+\u00a0F1534C and V1016G\u00a0+\u00a0F1534C were also confirmed in both countries, and their frequency was high (&gt;90%) in Phnom Penh, Cambodia. Strains having these alleles exhibited substantially higher levels of pyrethroid resistance than any other field population ever reported. The L982W substitution has never been detected in any country of the Indochina Peninsula except Vietnam and Cambodia, but it may be spreading to other areas of Asia, which can cause an unprecedentedly serious threat to the control of dengue fever as well as other\n                    <jats:italic>Aedes<\/jats:italic>\n                    -borne infectious diseases.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.abq7345","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T13:58:26Z","timestamp":1671631106000},"source":"Crossref","is-referenced-by-count":60,"title":["Discovery of super\u2013insecticide-resistant dengue mosquitoes in Asia: Threats of concomitant knockdown resistance mutations"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3837-6551","authenticated-orcid":true,"given":"Shinji","family":"Kasai","sequence":"first","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1300-9883","authenticated-orcid":true,"given":"Kentaro","family":"Itokawa","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8597-6704","authenticated-orcid":true,"given":"Nozomi","family":"Uemura","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5885-2121","authenticated-orcid":true,"given":"Aki","family":"Takaoka","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Furutani","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihide","family":"Maekawa","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5735-8404","authenticated-orcid":true,"given":"Daisuke","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nozomi","family":"Imanishi-Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0416-2878","authenticated-orcid":true,"given":"Michael","family":"Amoa-Bosompem","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3053-9504","authenticated-orcid":true,"given":"Katsunori","family":"Murota","sequence":"additional","affiliation":[{"name":"Kagoshima Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Kagoshima 891-0105, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Higa","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1565-7596","authenticated-orcid":true,"given":"Hitoshi","family":"Kawada","sequence":"additional","affiliation":[{"name":"Department of Vector Ecology and Environment, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noboru","family":"Minakawa","sequence":"additional","affiliation":[{"name":"Department of Vector Ecology and Environment, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0721-6248","authenticated-orcid":true,"given":"Tran Chi","family":"Cuong","sequence":"additional","affiliation":[{"name":"Medical Entomology and Zoology Department, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi","family":"Yen","sequence":"additional","affiliation":[{"name":"Medical Entomology and Zoology Department, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Vu","family":"Phong","sequence":"additional","affiliation":[{"name":"Medical Entomology and Zoology Department, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sath","family":"Keo","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Royal University of Agriculture, P.O. Box 2696, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6818-1982","authenticated-orcid":true,"given":"Kroesna","family":"Kang","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Royal University of Agriculture, P.O. Box 2696, Phnom Penh, Cambodia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3233-0202","authenticated-orcid":true,"given":"Kozue","family":"Miura","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2413-3863","authenticated-orcid":true,"given":"Lee Ching","family":"Ng","sequence":"additional","affiliation":[{"name":"Environmental Health Institute, National Environment Agency, Singapore 138667, Singapore."},{"name":"School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwa-Jen","family":"Teng","sequence":"additional","affiliation":[{"name":"Center for Diagnostics and Vaccine Development, Centers for Disease Control, Ministry of Health and Welfare, Taipei City 10050, Taiwan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Dadzie","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, P.O. Box LG 581, Legon,, Ghana."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4816-041X","authenticated-orcid":true,"given":"Sri","family":"Subekti","sequence":"additional","affiliation":[{"name":"Entomology Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-3031","authenticated-orcid":true,"given":"Kris Cahyo","family":"Mulyatno","sequence":"additional","affiliation":[{"name":"Entomology Study Group, Institute of Tropical Disease, Universitas Airlangga, Surabaya 60115, Indonesia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Sawabe","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5029-3169","authenticated-orcid":true,"given":"Takashi","family":"Tomita","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8175-6402","authenticated-orcid":true,"given":"Osamu","family":"Komagata","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization Global strategy for dengue prevention and control 2012\u20132020 Geneva Switzerland (2012)."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1618558114"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1407-9"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0471-x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2968"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5462.2474"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49660-6"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2016.03.005"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1584\/jpestics.10.141"},{"key":"e_1_3_3_12_2","first-page":"61","article-title":"Cloning and sequencing of the para-type sodium channel gene from susceptible and kdr-resistant German cockroaches (Blattella germanica) and house fly (Musca domestica)","volume":"252","author":"Miyazaki M.","year":"1996","unstructured":"M.\u00a0Miyazaki, K.\u00a0Ohyama, D. Y.\u00a0Dunlap, F.\u00a0Matsumura, Cloning and sequencing of the para-type sodium channel gene from susceptible and kdr-resistant German cockroaches (Blattella germanica) and house fly (Musca domestica). Mol. Gen. Genet. 252, 61\u201368 (1996).","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000527"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6324"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2003.00412.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008154"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009606"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003032"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3472-1"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21686"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003085"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0233669"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2512.181193"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/1010539517753876"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05156-3"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2019.24.5.1700847"},{"key":"e_1_3_3_27_2","unstructured":"C.\u00a0K.\u00a0Lok Singapore\u2019s Dengue Haemorrhagic Fever Control Programme: A Case Study on the Successful Control of Aedes aegypti and Aedes albopictus Using Mainly Environmental Measures as a Part of Integrated Vector Control (Southeast Asian Medical Information Center Tokyo 1985) pp.\u00a0114."},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.64.217"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305118110"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5763"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4771"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2010.02717.x"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.03.012"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2016.06.009"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12605"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2899-0"},{"key":"e_1_3_3_37_2","unstructured":"J. Hustedt thesis London School of Hygiene & Tropical Medicine (2020)."},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjaa238"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000391"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/s41182-021-00321-3"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/mve.12580"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04090-6"},{"key":"e_1_3_3_43_2","first-page":"64","article-title":"Presumption of the origin of Aedes aegypti discovered at CHUBU Centrair International Airport, Nagoya","volume":"70","author":"Hu J.","year":"2019","unstructured":"J.\u00a0Hu, K.\u00a0Itokawa, Y.\u00a0Tsuda, K.\u00a0Futami, Y.\u00a0Higa, K.\u00a0Sawabe, N.\u00a0Minakawa, Presumption of the origin of Aedes aegypti discovered at CHUBU Centrair International Airport, Nagoya. Med. Entomol. Zool. 70, 64 (2019).","journal-title":"Med. Entomol. Zool."},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.09.005"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2096-6"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009549"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12206"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002948"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-67"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11357-z"},{"key":"e_1_3_3_51_2","article-title":"Probit analysis","volume":"60","author":"Finney D. J.","year":"1971","unstructured":"D. J.\u00a0Finney, Probit analysis. J. Pharm. Sci. 60, (1971).","journal-title":"J. Pharm. Sci."},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/jvec.12298"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007818"},{"key":"e_1_3_3_54_2","unstructured":"P.\u00a0H.\u00a0A. Sneath R.\u00a0R.\u00a0Sokal Numerical Taxonomy: The Principles and Practice of Numerical Classification (W.H. Freeman and Co. 973) pp.\u00a0573."},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau2596"},{"key":"e_1_3_3_59_2","first-page":"Unit\u20135.6","article-title":"Comparative protein structure modeling using MODELLER","volume":"5","author":"Eswar N.","year":"2016","unstructured":"N.\u00a0Eswar, B.\u00a0Webb, M. A.\u00a0Marti-Renom, M. S.\u00a0Madhusudhan, D.\u00a0Eramian, M.-Y.\u00a0Shen, U.\u00a0Pieper, A.\u00a0Sali, Comparative protein structure modeling using MODELLER. Curr. Protoc. Bioinformatics 5, Unit\u20135.6 (2016).","journal-title":"Curr. Protoc. Bioinformatics"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(19981115)19:14<1639::AID-JCC10>3.0.CO;2-B"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.abq7345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T17:34:37Z","timestamp":1704821677000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.abq7345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,23]]},"references-count":59,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2022,12,23]]}},"alternative-id":["10.1126\/sciadv.abq7345"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.abq7345","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.03.05.483084","asserted-by":"object"}]},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,23]]},"article-number":"eabq7345"}}