{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:22:33Z","timestamp":1781760153895,"version":"3.54.5"},"reference-count":46,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"2","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,1,13]]},"abstract":"<jats:p>Kaposi\u2019s sarcoma (KS) is an endothelial cancer caused by the Kaposi\u2019s sarcoma-associated herpesvirus (KSHV) and is one of the most common cancers in sub-Saharan Africa. In limited-resource settings, traditional pathology infrastructure is often insufficient for timely diagnosis, leading to frequent diagnoses at advanced-stage disease where survival is poor. In this study, we investigate molecular diagnosis of KS performed in a point-of-care device to circumvent the limited infrastructure for traditional diagnosis. Using 506 mucocutaneous biopsies collected from patients at three HIV clinics in Uganda, we achieved 97% sensitivity, 92% specificity, and 96% accuracy compared to gold standard U.S.-based pathology. The results presented in this manuscript show that LAMP-based quantification of KSHV DNA extracted from KS-suspected biopsies has the potential to serve as a successful diagnostic for the disease and that diagnosis may be accurately achieved using a point-of-care device, reducing the barriers to obtaining KS diagnosis while increasing diagnostic accuracy.<\/jats:p>","DOI":"10.1126\/sciadv.adc8913","type":"journal-article","created":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T18:59:11Z","timestamp":1673636351000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":10,"title":["LAMP-enabled diagnosis of Kaposi\u2019s sarcoma for sub-Saharan Africa"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7738-0142","authenticated-orcid":true,"given":"Duncan","family":"McCloskey","sequence":"first","affiliation":[{"name":"Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aggrey","family":"Semeere","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Racheal","family":"Ayanga","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Laker-Oketta","sequence":"additional","affiliation":[{"name":"Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9449-6649","authenticated-orcid":true,"given":"Robert","family":"Lukande","sequence":"additional","affiliation":[{"name":"Pathology Department, Makerere University College of Health Sciences, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Semakadde","sequence":"additional","affiliation":[{"name":"HIV Clinic, Masaka Regional Referral Hospital, Masaka, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1936-7681","authenticated-orcid":true,"given":"Micheal","family":"Kanyesigye","sequence":"additional","affiliation":[{"name":"Immune Suppression Syndrome Clinic, Mbarara Regional Referral Hospital, Mbarara, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Wenger","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics, University of California, San\u00a0Francisco, San\u00a0Francisco, CA 94143, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"LeBoit","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, University of California, San\u00a0Francisco, San\u00a0Francisco, CA 94143, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"McCalmont","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, University of California, San\u00a0Francisco, San\u00a0Francisco, CA 94143, USA."},{"name":"Golden State Dermatology Dermatopathology, Walnut Creek, CA 94598, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toby","family":"Maurer","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN 46202, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5572-1647","authenticated-orcid":true,"given":"Andrea","family":"Gardner","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, Weill Cornell Medical College; 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