{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:42:31Z","timestamp":1793385751227,"version":"4.5.0"},"reference-count":46,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"1","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,1,6]]},"abstract":"<jats:p>\n                    Cell therapies and regenerative medicine interventions require an adequate source of therapeutic cells. Here, we demonstrate that constructing in vivo osteo-organoids by implanting bone morphogenetic protein\u20132\u2013loaded scaffolds into the internal muscle pocket near the femur of mice supports the growth and subsequent harvest of therapeutically useful cells including hematopoietic stem\/progenitor cells (HSPCs), mesenchymal stem cells (MSCs), lymphocytes, and myeloid cells. Profiling of the in vivo osteo-organoid maturation process delineated three stages\u2014fibroproliferation, osteochondral differentiation, and marrow generation\u2014each of which entailed obvious changes in the organoid structure and cell type distribution. The MSCs harvested from the osteochondral differentiation stage mitigated carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2013induced chronic liver fibrosis in mice, while HSPCs and immune cells harvested during the marrow generation stage rapidly and effectively reconstituted the impaired peripheral and solid immune organs of irradiated mice. These findings demonstrate the therapeutic potentials of in vivo osteo-organoid\u2013derived cells in cell therapies.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.add1541","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T16:36:19Z","timestamp":1674232579000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":45,"title":["A BMP-2\u2013triggered in vivo osteo-organoid for cell therapy"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6551-8463","authenticated-orcid":true,"given":"Kai","family":"Dai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China."},{"name":"Engineering Research Center for Biomedical Materials of the Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China."},{"name":"Frontiers 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