{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:48:22Z","timestamp":1789094902661,"version":"build-2803163510"},"reference-count":57,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"49","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2022,12,9]]},"abstract":"<jats:p>\n            To adapt to changes in environmental cues,\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            produces an array of virulence factors to survive the host immune responses during infection. Metabolic products contribute to bacterial virulence; however, only a limited number of these signaling receptors have been explored in detail for their ability to modulate virulence in bacteria. Here, we characterize the metabolic pathway of 2-methylcitrate cycle in\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            and unveil that PmiR served as a receptor of 2-methylisocitrate (MIC) to govern bacterial virulence. Crystallographic studies and structural-guided mutagenesis uncovered several residues crucial for PmiR\u2019s allosteric activation by MIC. We also demonstrated that PmiR directly repressed the\n            <jats:italic>pqs<\/jats:italic>\n            quorum-sensing system and subsequently inhibited pyocyanin production. Moreover, mutation of\n            <jats:italic>pmiR<\/jats:italic>\n            reduces bacterial survival in a mouse model of acute pneumonia infection. Collectively, this study identified\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            PmiR as an important metabolic sensor for regulating expression of bacterial virulence genes to adapt to the harsh environments.\n          <\/jats:p>","DOI":"10.1126\/sciadv.add4220","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T18:58:37Z","timestamp":1670439517000},"source":"Crossref","is-referenced-by-count":22,"title":["PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in\n            <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1126","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0599-828X","authenticated-orcid":true,"given":"Guoyan","family":"Cui","sequence":"first","affiliation":[{"name":"Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi\u2019an, ShaanXi, 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