{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:25:34Z","timestamp":1787016334179,"version":"build-2736575974"},"reference-count":53,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"10","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,3,10]]},"abstract":"<jats:p>Mouse esophagus is lined with a stratified epithelium, which is maintained by the constant renewal of unipotent progenitors. In this study, we profiled mouse esophagus by single-cell RNA sequencing and found taste buds specifically in the cervical segment of the esophagus. These taste buds have the same cellular composition as the ones from the tongue but express fewer taste receptor types. State-of-the-art transcriptional regulatory network analysis allowed the identification of specific transcription factors associated to the differentiation of immature progenitors into the three different taste bud cell types. Lineage tracing experiments revealed that esophageal taste buds arise from squamous bipotent progenitor, thus demonstrating that all esophageal progenitors are not unipotent. Our cell resolution characterization of cervical esophagus epithelium will enable a better understanding of esophageal progenitor potency and insights into the mechanisms involved in the development of taste buds.<\/jats:p>","DOI":"10.1126\/sciadv.add9135","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T13:57:16Z","timestamp":1678283836000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":21,"title":["Single-cell transcriptomics uncovers the differentiation of a subset of murine esophageal progenitors into taste buds in vivo"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6365-3418","authenticated-orcid":true,"given":"Aliz\u00e9e","family":"Vercauteren Drubbel","sequence":"first","affiliation":[{"name":"IRIBHM, ULB\/ Faculty of medicine, 808 Route de Lennik, 1070 Brussels, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8162-7566","authenticated-orcid":true,"given":"Benjamin","family":"Beck","sequence":"additional","affiliation":[{"name":"IRIBHM, ULB\/ Faculty of medicine, 808 Route de Lennik, 1070 Brussels, Belgium."},{"name":"Welbio\/FNRS Principal investigator at IRIBHM, 808 Route de Lennik, 1070 Brussels, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001170106"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0421"},{"key":"e_1_3_3_4_2","first-page":"3860","article-title":"A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification","volume":"118","author":"Kalabis J.","year":"2008","unstructured":"J.\u00a0Kalabis, K.\u00a0Oyama, T.\u00a0Okawa, H.\u00a0Nakagawa, C. Z.\u00a0Michaylira, D. B.\u00a0Stairs, J.-L.\u00a0Figueiredo, U.\u00a0Mahmood, J. A.\u00a0Diehl, M.\u00a0Herlyn, A. K.\u00a0Rustgi, A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification. J. Clin. Invest. 118, 3860\u20133869 (2008).","journal-title":"J. Clin. Invest."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.09.027"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI88941"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218835"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0611"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201003144"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.27.2.263"},{"key":"e_1_3_3_11_2","first-page":"349","article-title":"Renewal of taste bud cells in rat circumvallate papillae","volume":"13","author":"Farbman A. I.","year":"1980","unstructured":"A. I.\u00a0Farbman, Renewal of taste bud cells in rat circumvallate papillae. Cell Tissue Kinet. 13, 349\u2013357 (1980).","journal-title":"Cell Tissue Kinet."},{"key":"e_1_3_3_12_2","first-page":"107","article-title":"Taste bud regeneration and the search for taste progenitor cells","volume":"148","author":"Miura H.","year":"2010","unstructured":"H.\u00a0Miura, L. A.\u00a0Barlow, Taste bud regeneration and the search for taste progenitor cells. Arch. Ital. Biol. 148, 107\u2013118 (2010).","journal-title":"Arch. Ital. Biol."},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1111\/j.1460-9568.2010.07184.x","article-title":"Stem and progenitor cell compartments within adult mouse taste buds","volume":"31","author":"Sullivan J. M.","year":"2010","unstructured":"J. M.\u00a0Sullivan, A. A.\u00a0Borecki, S.\u00a0Oleskevich, Stem and progenitor cell compartments within adult mouse taste buds. Eur.\u00a0J.\u00a0Neurosci. 31, 1549\u20131560 (2010).","journal-title":"Eur.\u00a0J.\u00a0Neurosci."},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.69.209"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24121"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(84)90142-2"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092270313"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00455.2016"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep09007"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23804"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0336-2"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.05.061"},{"key":"e_1_3_3_24_2","unstructured":"D.\u00a0E.\u00a0Haines G.\u00a0A.\u00a0Mihailoff Fundamental Neuroscience for Basic and Clinical Applications (Elsevier 2018)."},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2820"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04099-5"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cophys.2021.01.003"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2963"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0267683"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.107631"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20731"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006990"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2021.03.019"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjh248"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1719109115"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.350"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901720405"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63855-7.00012-5"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-32948-0_1"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2015.10.007"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00455-019-10051-8"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2005.04.039"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.11R08CP"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.004"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/69.supplement_1.2S"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.099"},{"key":"e_1_3_3_47_2","unstructured":"D. Powell M.\u00a0Milton A.\u00a0Perry K.\u00a0Santos drpowell\/degust 4.1.1 (Zenodo 2019)."},{"key":"e_1_3_3_48_2","unstructured":"K. Blighe kevinblighe\/EnhancedVolcano (2020)."},{"key":"e_1_3_3_49_2","first-page":"460147","article-title":"Comprehensive integration of single cell data","author":"Stuart T.","year":"2018","unstructured":"T.\u00a0Stuart, A.\u00a0Butler, P.\u00a0Hoffman, C.\u00a0Hafemeister, E.\u00a0Papalexi, W. M.\u00a0Mauck, M.\u00a0Stoeckius, P.\u00a0Smibert, R.\u00a0Satija, Comprehensive integration of single cell data. bioRxiv , 460147 (2018).","journal-title":"bioRxiv"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4292"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2859"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa099"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14766-3"},{"key":"e_1_3_3_54_2","unstructured":"J.\u00a0C.\u00a0Oliveros VENNY. An Interactive Tool for Comparing Lists With Venn\u2019s Diagrams. (2007); https:\/\/bioinfogp.cnb.csic.es\/tools\/venny\/index.html."}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.add9135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T16:36:08Z","timestamp":1704818168000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.add9135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,10]]},"references-count":53,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,3,10]]}},"alternative-id":["10.1126\/sciadv.add9135"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.add9135","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,10]]},"assertion":[{"value":"2022-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadd9135"}}