{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:39:17Z","timestamp":1789785557599,"version":"4.0.1"},"reference-count":63,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"2","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,1,13]]},"abstract":"<jats:p>\n            Parvovirus B19 (B19V) infects human erythroid progenitor cells (EPCs) and causes several hematological disorders and fetal hydrops. Amino acid (aa) 5\u201368 of minor capsid protein VP1 (VP1u\n            <jats:sup>5\u201368aa<\/jats:sup>\n            ) is the minimal receptor binding domain for B19V to enter EPCs. Here, we carried out a genome-wide CRISPR-Cas9 guide RNA screen and identified tyrosine protein kinase receptor UFO (AXL) as a proteinaceous receptor for B19V infection of EPCs.\n            <jats:italic>AXL<\/jats:italic>\n            gene silencing in ex vivo expanded EPCs remarkably decreased B19V internalization and replication. Additions of the recombinant AXL extracellular domain or a polyclonal antibody against it upon infection efficiently inhibited B19V infection of ex vivo expanded EPCs. Moreover, B19V VP1u interacted with the recombinant AXL extracellular domain in vitro at a relatively high affinity (\n            <jats:italic>K<\/jats:italic>\n            <jats:sub>D<\/jats:sub>\n            \u00a0=\u00a0103 nM). Collectively, we provide evidence that AXL is a co-receptor for B19V infection of EPCs.\n          <\/jats:p>","DOI":"10.1126\/sciadv.ade0869","type":"journal-article","created":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T18:58:52Z","timestamp":1673463532000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":37,"title":["Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1654-9770","authenticated-orcid":true,"given":"Kang","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8292-5127","authenticated-orcid":true,"given":"Wei","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4886-2120","authenticated-orcid":true,"given":"Peng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"GeneGoCell Inc., San Diego, CA 92127, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Zhang-Chen","sequence":"additional","affiliation":[{"name":"GeneGoCell Inc., San Diego, CA 92127, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5942-2118","authenticated-orcid":true,"given":"Steve","family":"Kleiboeker","sequence":"additional","affiliation":[{"name":"Department of Research and Development, ViraCor Eurofins Laboratories, Lenexa, KS 66219, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9850-1695","authenticated-orcid":true,"given":"Jianming","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001212"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00040-16"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)92739-2"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)91579-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra030840"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/593190"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","unstructured":"B.\u00a0E.\u00a0Torbett J.\u00a0S.\u00a0Friedman Erythropoiesis: An overview in Erythropoietins Erythropoietic Factors and Erythropoiesis S.\u00a0G.\u00a0Elliott M.\u00a0A.\u00a0Foote G.\u00a0Molineux Eds. 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