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We have created an extracellular vesicle\u2013based technology that allows viral membrane antigens to be selectively recruited onto the surface of WW domain\u2013activated extracellular vesicles (WAEVs). Budding of WAEVs requires secretory carrier-associated membrane protein 3, which through its proline-proline-alanine-tyrosine motif interacts with WW domains to recruit fused viral membrane antigens onto WAEVs. Immunization with influenza and HIV viral membrane proteins displayed on WAEVs elicits production of virus-specific neutralizing antibodies and, in the case of influenza antigens, protects mice from the lethal viral infection. WAEVs thus represent a versatile platform for presenting and delivering membrane antigens as vaccines against influenza, HIV, and potentially many other viral pathogens.<\/jats:p>","DOI":"10.1126\/sciadv.ade2708","type":"journal-article","created":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T19:02:24Z","timestamp":1674846144000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":24,"title":["Displaying and delivering viral membrane antigens via WW domain\u2013activated extracellular vesicles"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0379-1701","authenticated-orcid":true,"given":"Sengjin","family":"Choi","sequence":"first","affiliation":[{"name":"Program in Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard T.H. 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