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In this study, we identify a cue within the developing T cell niche that is critical for T cell development. E-cadherin mediates cell-cell interactions and influences cell fate in many developmental systems. In developing T cells, E-cadherin contributed to the formation of an immunological synapse and the alignment of the mitotic spindle with the polarity axis during division, which facilitated subsequent T cell development. Collectively, these data suggest that E-cadherin facilitates interactions with the thymic niche to coordinate the \u03b2-selection stage of T cell development.<\/jats:p>","DOI":"10.1126\/sciadv.ade5348","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T18:58:54Z","timestamp":1674068334000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":7,"title":["E-cadherin in developing murine T cells controls spindle alignment and progression through \u03b2-selection"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3845-9290","authenticated-orcid":true,"given":"Mirren","family":"Charnley","sequence":"first","affiliation":[{"name":"Optical Sciences Centre, School of Science, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia."},{"name":"Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amr H.","family":"Allam","sequence":"additional","affiliation":[{"name":"Optical Sciences Centre, School of Science, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia."},{"name":"Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5984-5778","authenticated-orcid":true,"given":"Lucas M.","family":"Newton","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia."},{"name":"Research Centre for Molecular Cancer Prevention, La Trobe University, Melbourne, Victoria 3086, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1366-6691","authenticated-orcid":true,"given":"Patrick O.","family":"Humbert","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia."},{"name":"Research Centre for Molecular Cancer Prevention, La Trobe University, Melbourne, Victoria 3086, Australia."},{"name":"Department of Biochemistry and Pharmacology, University of Melbourne, Melbourne, Victoria 3010, Australia."},{"name":"Department of Clinical Pathology, University of Melbourne, Melbourne, Victoria 3010, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5826-9641","authenticated-orcid":true,"given":"Sarah M.","family":"Russell","sequence":"additional","affiliation":[{"name":"Optical Sciences Centre, School of Science, Swinburne University of Technology, Hawthorn, Victoria 3122, Australia."},{"name":"Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, Victoria 3000, Australia."},{"name":"Sir Peter MacCallum Department of Oncology, The University of Melbourne, Victoria 3010, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302259"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST20180414"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-5323(02)00064-7"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2021.06.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201908108"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.235358"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1234"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091430"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20121412"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13317"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108967"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5365.905"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1504971112"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201502053"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2201907119"},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","unstructured":"J.\u00a0S.\u00a0Duke-Cohan A.\u00a0Akitsu R.\u00a0J.\u00a0Mallis C.\u00a0M.\u00a0Messier P.\u00a0H.\u00a0Lizotte W.\u00a0Hwang M.\u00a0J.\u00a0Lang E.\u00a0L.\u00a0Reinherz Pre-T cell receptor self-MHC sampling restricts thymocyte dedifferentiation. 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