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However, immunologic interactions of this xenogeneic platform have not been characterized, thus limiting potential translational applications. Using flow cytometry and immunohistochemistry, we demonstrate that porcine immune cell and immunoglobulin infiltration occurs in this xenogeneic XC system, in the context of calcineurin-based immunosuppression and complement depletion. Despite this, xenogeneic XC supported the viability, tissue integrity, and physiologic improvement of human donor lungs over 24 hours of xeno-support. These findings provide targets for future immunomodulatory strategies to minimize immunologic interactions on this organ support biotechnology.<\/jats:p>","DOI":"10.1126\/sciadv.ade7647","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T17:59:37Z","timestamp":1680285577000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":11,"title":["Immune characterization of a xenogeneic human lung cross-circulation support system"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1834-5931","authenticated-orcid":true,"given":"Wei K.","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, TN, USA."},{"name":"Department of Surgery, Division of Hepatobiliary Surgery and Liver Transplantation, Vanderbilt University Medical Center, Nashville, TN, 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