{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:00:08Z","timestamp":1789520408985,"version":"build-2803163510"},"reference-count":59,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"27","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,7,7]]},"abstract":"<jats:p>\n                    Malaria is among the world\u2019s deadliest diseases, predominantly affecting Sub-Saharan Africa and killing over half a million people annually. Controlling the principal vector, the mosquito\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    , as well as other anophelines, is among the most effective methods to control disease spread. Here, we develop a genetic population suppression system termed Ifegenia (inherited female elimination by genetically encoded nucleases to interrupt alleles) in this deadly vector. In this bicomponent CRISPR-based approach, we disrupt a female-essential gene,\n                    <jats:italic>femaleless<\/jats:italic>\n                    (\n                    <jats:italic>fle<\/jats:italic>\n                    ), demonstrating complete genetic sexing via heritable daughter gynecide. Moreover, we demonstrate that Ifegenia males remain reproductively viable and can load both\n                    <jats:italic>fle<\/jats:italic>\n                    mutations and CRISPR machinery to induce\n                    <jats:italic>fle<\/jats:italic>\n                    mutations in subsequent generations, resulting in sustained population suppression. Through modeling, we demonstrate that iterative releases of nonbiting Ifegenia males can act as an effective, confinable, controllable, and safe population suppression and elimination system.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ade8903","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T13:57:39Z","timestamp":1688565459000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":35,"title":["A confinable female-lethal population suppression system in the malaria vector,\n                    <i>Anopheles gambiae<\/i>"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3281-1161","authenticated-orcid":true,"given":"Andrea L.","family":"Smidler","sequence":"first","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7247-5142","authenticated-orcid":true,"given":"James J.","family":"Pai","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2059-4614","authenticated-orcid":true,"given":"Reema A.","family":"Apte","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7378-8853","authenticated-orcid":true,"given":"H\u00e9ctor M.","family":"S\u00e1nchez C.","sequence":"additional","affiliation":[{"name":"Divisions of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8929-2200","authenticated-orcid":true,"given":"Rodrigo M.","family":"Corder","sequence":"additional","affiliation":[{"name":"Divisions of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4027-1388","authenticated-orcid":true,"given":"Eileen","family":"Jeffrey Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Divisions of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, CA 94720, USA."},{"name":"Oxitec Ltd., Abingdon, OX14 4RQ, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5842-8136","authenticated-orcid":true,"given":"Neha","family":"Thakre","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9934-3040","authenticated-orcid":true,"given":"Igor","family":"Antoshechkin","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering (BBE), California Institute of Technology, Pasadena, CA 91125, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0603-7341","authenticated-orcid":true,"given":"John M.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Divisions of Epidemiology and Biostatistics, School of Public Health, University of California, Berkeley, CA 94720, USA."},{"name":"Innovative Genomics Institute, University of California, Berkeley, CA 94720, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6853-9884","authenticated-orcid":true,"given":"Omar S.","family":"Akbari","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","unstructured":"World\u00a0Health\u00a0Organization Others World malaria report 2020: 20 years of global progress and challenges (2020); 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M.\u00a0Loh, M.\u00a0Su, C.\u00a0Montell, J.\u00a0Marshall, Targeting sex determination to suppress mosquito populations. Res. Sq, rs-2834069 (2023).","journal-title":"Res. Sq"},{"key":"e_1_3_2_23_2","first-page":"20180776","article-title":"Self-limiting population genetic control with sex-linked genome editors","volume":"285","author":"Burt A.","year":"2018","unstructured":"A.\u00a0Burt, A.\u00a0Deredec, Self-limiting population genetic control with sex-linked genome editors. Proc. Biol. Sci. 285, 20180776 (2018).","journal-title":"Proc. Biol. 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P.-S.\u00a0Chan, A quantitative model-independent method for global sensitivity analysis of model output. Dent. Tech. 41, 39\u201356 (1999).","journal-title":"Dent. 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