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Human innate immune macrophages were verified as essential to biomaterial rejection in this model and were capable of cross-talk with mouse fibroblasts for collagen matrix deposition. Cytokine and cytokine receptor array analysis confirmed core signaling in the fibrotic cascade. Foreign body giant cell formation, often unobserved in mice, was also prominent. Last, high-resolution microscopy coupled with multiplexed antibody capture digital profiling analysis supplied spatial resolution of rejection responses. This model enables the study of human immune cell\u2013mediated fibrosis and interactions with implanted biomaterials and devices.<\/jats:p>","DOI":"10.1126\/sciadv.ade9488","type":"journal-article","created":{"date-parts":[[2023,6,16]],"date-time":"2023-06-16T17:58:40Z","timestamp":1686938320000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":26,"title":["Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4323-3264","authenticated-orcid":true,"given":"Joshua C.","family":"Doloff","sequence":"first","affiliation":[{"name":"David H.\u00a0Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 500 Main St., Cambridge, MA 02139, USA."},{"name":"Department of Anesthesiology, Boston Children\u2019s Hospital, 300 Longwood Ave., Boston, MA 02115, 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