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By integrating image segmentation, three-dimensional reconstruction, and deep-learning approaches, we found and characterized anatomically complex intercellular connections bridging pairs of cerebellar granule cells throughout the EGL. Connected cells were either mitotic, migratory, or transitioning between these two cell stages, displaying a chronological continuum of proliferative and migratory events never previously observed in vivo at this resolution. This unprecedented ultrastructural characterization poses intriguing hypotheses about intercellular connectivity between developing progenitors and its possible role in the development of the central nervous system.<\/jats:p>","DOI":"10.1126\/sciadv.adf3471","type":"journal-article","created":{"date-parts":[[2023,4,5]],"date-time":"2023-04-05T17:58:37Z","timestamp":1680717517000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":22,"title":["3D reconstruction of the cerebellar germinal layer reveals tunneling connections between developing granule cells"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3885-0794","authenticated-orcid":true,"given":"Di\u00e9go","family":"Cordero Cervantes","sequence":"first","affiliation":[{"name":"Membrane Traffic and Pathogenesis, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3691, F-75015 Paris, France."},{"name":"Universit\u00e9 Paris-Saclay, 91405 Orsay, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8131-0542","authenticated-orcid":true,"given":"Harshavardhan","family":"Khare","sequence":"additional","affiliation":[{"name":"Membrane Traffic and Pathogenesis, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3691, F-75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa Michelle","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Neurology, Department of Psychiatry, Icahn School of Medicine at Mount Sinai, New\u00a0York, NY 10029, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6093-0387","authenticated-orcid":true,"given":"Nathaly Dongo","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Membrane Traffic and Pathogenesis, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3691, F-75015 Paris, France."},{"name":"Research Center in Bioengineering, Universidad de Ingenier\u00eda y Tecnolog\u00eda-UTEC, Lima 15049, Peru."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orfane","family":"Coulon-Mahdi","sequence":"additional","affiliation":[{"name":"Membrane Traffic and Pathogenesis, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3691, F-75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0208-3212","authenticated-orcid":true,"given":"Jeff William","family":"Lichtman","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, Center for Brain Science, Harvard University, Cambridge, MA 02138, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6048-6602","authenticated-orcid":true,"given":"Chiara","family":"Zurzolo","sequence":"additional","affiliation":[{"name":"Membrane Traffic and Pathogenesis, Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 3691, F-75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03778-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(95)90236-8"},{"key":"e_1_3_3_4_2","unstructured":"C. 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