{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:29:06Z","timestamp":1791862146445,"version":"4.3.4"},"reference-count":22,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"38","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,9,22]]},"abstract":"<jats:p>Measurable residual disease (MRD), defined as the population of cancer cells that persist following therapy, serves as the critical reservoir for disease relapse in acute myeloid leukemia and other malignancies. Understanding the biology enabling MRD clones to resist therapy is necessary to guide the development of more effective curative treatments. Discriminating between residual leukemic clones, preleukemic clones, and normal precursors remains a challenge with current MRD tools. Here, we developed a single-cell MRD (scMRD) assay by combining flow cytometric enrichment of the targeted precursor\/blast population with integrated single-cell DNA sequencing and immunophenotyping. Our scMRD assay shows high sensitivity of approximately 0.01%, deconvolutes clonal architecture, and provides clone-specific immunophenotypic data. In summary, our scMRD assay enhances MRD detection and simultaneously illuminates the clonal architecture of clonal hematopoiesis\/preleukemic and leukemic cells surviving acute myeloid leukemia therapy.<\/jats:p>","DOI":"10.1126\/sciadv.adg0488","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T13:59:54Z","timestamp":1695218394000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":54,"title":["Single-cell genotypic and phenotypic analysis of measurable residual disease in acute myeloid leukemia"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5807-4465","authenticated-orcid":true,"given":"Troy M.","family":"Robinson","sequence":"first","affiliation":[{"name":"Human Oncology and Pathogenesis Program, Molecular Cancer Medicine Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."},{"name":"Louis V.\u00a0Gerstner Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8294-8748","authenticated-orcid":true,"given":"Robert L.","family":"Bowman","sequence":"additional","affiliation":[{"name":"Human Oncology and Pathogenesis Program, Molecular Cancer Medicine Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonali","family":"Persaud","sequence":"additional","affiliation":[{"name":"Human Oncology and Pathogenesis Program, Molecular Cancer Medicine Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Hematopathology Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."},{"name":"Department of Pathology and Laboratory Medicine, Molecular Diagnostic Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Neigenfind","sequence":"additional","affiliation":[{"name":"Human Oncology and Pathogenesis Program, Molecular Cancer Medicine Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Hematopathology Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Hematopathology Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, 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Medicine, Hematopathology Service, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, USA."},{"name":"Center for Hematologic Malignancies, Memorial Sloan Kettering Cancer Center, New\u00a0York, NY, 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Jacobsen B.\u00a0Psaila S.\u00a0Thongjuea I.\u00a0Antony-Debr\u00e9 A.\u00a0J.\u00a0Mead Deciphering TP53 mutant cancer evolution with single-cell multi-omics. bioRxiv 2022.03.28.485984 [Preprint] (2022). https:\/\/doi.org\/10.1101\/2022.03.28.485984.","DOI":"10.1101\/2022.03.28.485984"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-2932"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.04.001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.09.004"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","unstructured":"S. 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