{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T10:43:12Z","timestamp":1786444992933,"version":"build-2736575974"},"reference-count":58,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"51","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,12,22]]},"abstract":"<jats:p>\n                    We report on the identification of extracellular miRNA (ex-miRNA) biomarkers for early diagnosis and prognosis of preeclampsia (PE). Small RNA sequencing of maternal serum prospectively collected from participants undergoing evaluation for suspected PE revealed distinct patterns of ex-miRNA expression among different categories of hypertensive disorders in pregnancy. Applying an iterative machine learning method identified three bivariate miRNA biomarkers (\n                    <jats:italic>miR-522-3p\/miR-4732-5p<\/jats:italic>\n                    ,\n                    <jats:italic>miR-516a-5p\/miR-144-3p<\/jats:italic>\n                    , and\n                    <jats:italic>miR-27b-3p\/let-7b-5p<\/jats:italic>\n                    ) that, when applied serially, distinguished between PE cases of different severity and differentiated cases from controls with a sensitivity of 93%, specificity of 79%, positive predictive value (PPV) of 55%, and negative predictive value (NPV) of 89%. In a small independent validation cohort, these ex-miRNA biomarkers had a sensitivity of 91% and specificity of 57%. Combining these ex-miRNA biomarkers with the established sFlt1:PlGF protein biomarker ratio performed better than either set of biomarkers alone (sensitivity of 89.4%, specificity of 91.3%, PPV of 95.5%, and NPV of 80.8%).\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adg7545","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T13:58:40Z","timestamp":1703080720000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":20,"title":["Discovery and verification of extracellular microRNA biomarkers for diagnostic and prognostic assessment of preeclampsia at triage"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8480-4564","authenticated-orcid":true,"given":"Robert","family":"Morey","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Medicine, University of California, San Diego, La Jolla, CA, 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