{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:40:18Z","timestamp":1790721618446,"version":"4.1.0"},"reference-count":70,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"22","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,5,31]]},"abstract":"<jats:p>\n                    Infusion of\n                    <jats:sup>13<\/jats:sup>\n                    C-labeled metabolites provides a gold standard for understanding the metabolic processes used by T cells during immune responses in vivo. Through infusion of\n                    <jats:sup>13<\/jats:sup>\n                    C-labeled metabolites (glucose, glutamine, and acetate) in\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    \u2013infected mice, we demonstrate that CD8 T effector (Teff) cells use metabolites for specific pathways during specific phases of activation. Highly proliferative early Teff cells in vivo shunt glucose primarily toward nucleotide synthesis and leverage glutamine anaplerosis in the tricarboxylic acid (TCA) cycle to support adenosine triphosphate and de novo pyrimidine synthesis. In addition, early Teff cells rely on glutamic-oxaloacetic transaminase 1 (Got1)\u2014which regulates de novo aspartate synthesis\u2014for effector cell expansion in vivo. CD8 Teff cells change fuel preference over the course of infection, switching from glutamine- to acetate-dependent TCA cycle metabolism late in infection. This study provides insights into the dynamics of Teff metabolism, illuminating distinct pathways of fuel consumption associated with CD8 Teff cell function in vivo.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adj1431","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T13:58:57Z","timestamp":1716991137000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":60,"title":["<sup>13<\/sup>\n                    C metabolite tracing reveals glutamine and acetate as critical in vivo fuels for CD8 T cells"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8556-980X","authenticated-orcid":true,"given":"Eric H.","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Metabolism and Nutritional Programming, Van Andel Institute, Grand Rapids, MI, 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