{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:33:01Z","timestamp":1783488781991,"version":"3.55.0"},"reference-count":68,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"9","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>\n            Wall teichoic acid (WTA), a covalent adduct of Gram-positive bacterial cell wall peptidoglycan, contributes directly to virulence and antibiotic resistance in pathogenic species. Polymerization of the\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            WTA ribitol-phosphate chain is catalyzed by TarL, a member of the largely uncharacterized TagF-like family of membrane-associated enzymes. We report the cryo\u2013electron microscopy structure of TarL, showing a tetramer that forms an extensive membrane-binding platform of monotopic helices. TarL is composed of an amino-terminal immunoglobulin-like domain and a carboxyl-terminal glycosyltransferase-B domain for ribitol-phosphate polymerization. The active site of the latter is complexed to donor substrate cytidine diphosphate\u2013ribitol, providing mechanistic insights into the catalyzed phosphotransfer reaction. Furthermore, the active site is surrounded by electropositive residues that serve to retain the lipid-linked acceptor for polymerization. Our data advance general insight into the architecture and membrane association of the still poorly characterized monotopic membrane protein class and present molecular details of ribitol-phosphate polymerization that may aid in the design of new antimicrobials.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adj3864","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T18:59:54Z","timestamp":1709146794000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":5,"title":["Cryo-EM analysis of\n            <i>S. aureus<\/i>\n            TarL, a polymerase in wall teichoic acid biogenesis central to virulence and antibiotic resistance"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7318-9754","authenticated-orcid":true,"given":"Franco K. 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DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7624-8112","authenticated-orcid":true,"given":"Eric D.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biomedical Sciences, Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4058-9425","authenticated-orcid":true,"given":"Natalie C. 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