{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T07:54:18Z","timestamp":1787644458773,"version":"build-2736575974"},"reference-count":47,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"49","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2023,12,8]]},"abstract":"<jats:p>\n            Secreted bacterial type III secretion system (T3SS) proteins are essential for successful infection by many human pathogens. Both T3SS translocator SipC and effector SipA are critical for\n            <jats:italic>Salmonella<\/jats:italic>\n            infection by subversion of the host cell cytoskeleton, but the precise molecular interplay between them remains unknown. Here, using cryo\u2013electron microscopy, we show that SipA binds along the F-actin grooves with a unique binding pattern. SipA stabilizes F-actin through charged interface residues and appears to prevent inorganic phosphate release through closure of the \u201cback door\u201d of adenosine 5\u2032-triphosphate pocket. We also show that SipC enhances the binding of SipA to F-actin, thus demonstrating that a sequential presence of T3SS proteins in host cells is associated with a sequence of infection events\u2014starting with actin nucleation, filament growth, and stabilization. Together, our data explain the coordinated interplay of a precisely tuned and highly effective mechanism during\n            <jats:italic>Salmonella<\/jats:italic>\n            infection and provide a blueprint for interfering with\n            <jats:italic>Salmonella<\/jats:italic>\n            effectors acting on actin.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adj5777","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T18:58:25Z","timestamp":1702061905000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":24,"title":["Structural basis for subversion of host cell actin cytoskeleton during\n            <i>Salmonella<\/i>\n            infection"],"prefix":"10.1126","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-6270","authenticated-orcid":true,"given":"Biao","family":"Yuan","sequence":"first","affiliation":[{"name":"University Medical Center Hamburg-Eppendorf (UKE), Institute of Structural and Systems Biology, Hamburg, Germany."},{"name":"Centre for Structural Systems Biology (CSSB), Hamburg, Germany."},{"name":"Deutsches Elektronen-Synchrotron Zentrum (DESY), Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4789-4249","authenticated-orcid":true,"given":"Jonas","family":"Scholz","sequence":"additional","affiliation":[{"name":"Institute for Biophysical Chemistry, Hannover Medical School, Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1539-6488","authenticated-orcid":true,"given":"Jiri","family":"Wald","sequence":"additional","affiliation":[{"name":"University Medical Center Hamburg-Eppendorf (UKE), Institute of Structural and Systems Biology, Hamburg, Germany."},{"name":"Centre for Structural Systems Biology (CSSB), Hamburg, Germany."},{"name":"Deutsches Elektronen-Synchrotron Zentrum (DESY), Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5369-1699","authenticated-orcid":true,"given":"Roland","family":"Thuenauer","sequence":"additional","affiliation":[{"name":"Centre for Structural Systems Biology (CSSB), Hamburg, Germany."},{"name":"Technology Platform Light Microscopy (TPLM), University of Hamburg, Hamburg, Germany."},{"name":"Technology Platform Microscopy and Image Analysis (TP MIA), Leibniz Institute of Virology (LIV), Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9009-9726","authenticated-orcid":true,"given":"Rory","family":"Hennell James","sequence":"additional","affiliation":[{"name":"University Medical Center Hamburg-Eppendorf (UKE), Institute of Structural and Systems Biology, Hamburg, Germany."},{"name":"Centre for Structural Systems Biology (CSSB), Hamburg, Germany."},{"name":"Deutsches Elektronen-Synchrotron Zentrum (DESY), Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Ellenberg","sequence":"additional","affiliation":[{"name":"University Medical Center Hamburg-Eppendorf (UKE), Institute for Biochemistry and Signal Transduction, Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Windhorst","sequence":"additional","affiliation":[{"name":"University Medical Center Hamburg-Eppendorf (UKE), Institute for Biochemistry and Signal Transduction, Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1803-9192","authenticated-orcid":true,"given":"Jan","family":"Faix","sequence":"additional","affiliation":[{"name":"Institute for Biophysical Chemistry, Hannover Medical School, Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8106-4038","authenticated-orcid":true,"given":"Thomas C.","family":"Marlovits","sequence":"additional","affiliation":[{"name":"University Medical Center Hamburg-Eppendorf (UKE), Institute of Structural and Systems Biology, Hamburg, Germany."},{"name":"Centre for Structural Systems Biology (CSSB), Hamburg, Germany."},{"name":"Deutsches Elektronen-Synchrotron Zentrum (DESY), Hamburg, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018226"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06247"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(04)00053-X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/45829"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00257"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.9.2131"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00877-19"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39514"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.18.4926"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.094045"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.05.065"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.286120"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.20"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00393-17"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1194598"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201802155"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1088433"},{"key":"e_1_3_2_19_2","first-page":"518","article-title":"The bacterial protein SipA polymerizes G-actin and mimics muscle nebulin","volume":"9","author":"Galkin V. E.","year":"2002","unstructured":"V. E.\u00a0Galkin, A.\u00a0Orlova, M. S.\u00a0VanLoock, D.\u00a0Zhou, J. E.\u00a0Gal\u00e1n, E. H.\u00a0Egelman, The bacterial protein SipA polymerizes G-actin and mimics muscle nebulin. Nat. Struct. Mol. Biol.9, 518\u2013521 (2002).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.086934"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002178"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503407102"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.02.001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab408"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019104006"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn1934"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2205370119"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-018-0128-3"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1915987117"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.11.065"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05241-8"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05366-w"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2004.00375.x"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2013.11.002"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1520040113"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.34"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)62016-2"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42166"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4193"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.08.008"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-019-0437-z"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-018-0074-0"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3943"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798318002425"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798319011471"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3330"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3541"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.adj5777","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T22:32:41Z","timestamp":1704839561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adj5777"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,8]]},"references-count":47,"journal-issue":{"issue":"49","published-print":{"date-parts":[[2023,12,8]]}},"alternative-id":["10.1126\/sciadv.adj5777"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.adj5777","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,8]]},"assertion":[{"value":"2023-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadj5777"}}