{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T02:47:49Z","timestamp":1785379669135,"version":"3.55.0"},"reference-count":45,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"34","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,8,23]]},"abstract":"<jats:p>\n            An outbreak of mpox virus in May 2022 has spread over 110 nonpandemic regions in the world, posing a great threat to global health. Mpox virus E5, a helicase-primase, plays an essential role in DNA replication, but the molecular mechanisms are elusive. Here, we report seven structures of mpox virus E5 in a double hexamer (DH) and six in single hexamer in different conformations, indicating a rotation mechanism for helicase and a coupling action for primase. The DH is formed through the interface of zinc-binding domains, and the central channel density indicates potential double-stranded DNA (dsDNA), which helps to identify dsDNA binding residues Arg\n            <jats:sup>249<\/jats:sup>\n            , Lys\n            <jats:sup>286<\/jats:sup>\n            , Lys\n            <jats:sup>315<\/jats:sup>\n            , and Lys\n            <jats:sup>317<\/jats:sup>\n            . Our work is important not only for understanding poxviral DNA replication but also for the development of novel therapeutics for serious poxviral infections including smallpox virus and mpox virus.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adl1150","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:27:22Z","timestamp":1724279242000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":7,"title":["Essential and multifunctional mpox virus E5 helicase-primase in double and single hexamer"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5430-0537","authenticated-orcid":true,"given":"Yunxia","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Thyroid and Breast Surgery, Zhongnan Hospital of Wuhan University, State Key Laboratory of Virology, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China."},{"name":"Key Laboratory 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