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The prM topology and domain assignment in TBEV is similar to the mosquito-borne Binjari virus, but is in contrast to other immature flavivirus models. These results support that prM cleavage, the collapse of E protein ectodomains onto the virion surface, the large movement of the membrane domains of both E and M, and the release of the pr fragment from the particle render the virus mature and infectious. Our work favors the collapse model of flavivirus maturation warranting further studies of immature flaviviruses to determine the sequence of events and mechanistic details driving flavivirus maturation.<\/jats:p>","DOI":"10.1126\/sciadv.adl1888","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T13:59:13Z","timestamp":1720015153000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":20,"title":["The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4555-6446","authenticated-orcid":true,"given":"Maria","family":"Anastasina","sequence":"first","affiliation":[{"name":"Faculty of Biological and Environmental Sciences, Molecular and Integrative Bioscience Research Programme, University of Helsinki, Helsinki, Finland."},{"name":"Helsinki Institute of Life 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