{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T08:43:24Z","timestamp":1789807404602,"version":"4.0.1"},"reference-count":99,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"30","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,7,26]]},"abstract":"<jats:p>\n                    The transitioning of neural stem cells (NSCs) between quiescent and proliferative states is fundamental for brain development and homeostasis. Defects in NSC reactivation are associated with neurodevelopmental disorders.\n                    <jats:italic>Drosophila<\/jats:italic>\n                    quiescent NSCs extend an actin-rich primary protrusion toward the neuropil. However, the function of the actin cytoskeleton during NSC reactivation is unknown. Here, we reveal the fine filamentous actin (F-actin) structures in the protrusions of quiescent NSCs by expansion and super-resolution microscopy. We show that F-actin polymerization promotes the nuclear translocation of myocardin-related transcription factor, a microcephaly-associated transcription factor, for NSC reactivation and brain development. F-actin polymerization is regulated by a signaling cascade composed of G protein\u2013coupled receptor Smog, G protein \u03b1\n                    <jats:sub>q<\/jats:sub>\n                    subunit, Rho1 guanosine triphosphatase, and Diaphanous (Dia)\/Formin during NSC reactivation. Further, astrocytes secrete a Smog ligand folded gastrulation to regulate G\u03b1\n                    <jats:sub>q<\/jats:sub>\n                    -Rho1-Dia\u2013mediated NSC reactivation. Together, we establish that the Smog-G\u03b1\n                    <jats:sub>q<\/jats:sub>\n                    -Rho1 signaling axis derived from astrocytes, an NSC niche, regulates Dia-mediated F-actin dynamics in NSC reactivation.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adl4694","type":"journal-article","created":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T13:59:07Z","timestamp":1721829547000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":9,"title":["Astrocytes control quiescent NSC reactivation via GPCR signaling\u2013mediated F-actin remodeling"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6197-3995","authenticated-orcid":true,"given":"Kun-Yang","family":"Lin","sequence":"first","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8702-4352","authenticated-orcid":true,"given":"Mahekta R.","family":"Gujar","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7229-4456","authenticated-orcid":true,"given":"Jiaen","family":"Lin","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei Yung","family":"Ding","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-2770","authenticated-orcid":true,"given":"Jiawen","family":"Huang","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6357-7470","authenticated-orcid":true,"given":"Yang","family":"Gao","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4840-1853","authenticated-orcid":true,"given":"Ye Sing","family":"Tan","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Teng","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, Level 5, T-lab Building, 5A Engineering Drive 1, Singapore, 117411, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Low Siok Lan","family":"Christine","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, Level 5, T-lab Building, 5A Engineering Drive 1, Singapore, 117411, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8765-3861","authenticated-orcid":true,"given":"Pakorn","family":"Kanchanawong","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, Level 5, T-lab Building, 5A Engineering Drive 1, Singapore, 117411, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3230-1062","authenticated-orcid":true,"given":"Yusuke","family":"Toyama","sequence":"additional","affiliation":[{"name":"Mechanobiology Institute, Level 5, T-lab Building, 5A Engineering Drive 1, Singapore, 117411, Singapore."},{"name":"Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, 117543, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-1878","authenticated-orcid":true,"given":"Hongyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Neuroscience and Behavioral Disorders Programme, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore."},{"name":"Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore."},{"name":"Integrative Sciences and Engineering Programme, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.01.001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2011.10.009"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12551"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008653"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.12.007"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09867"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/cen.12555"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1700-08.2009"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/417039a"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(02)00277-8"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00172-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.05.023"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2018.08.014"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000276"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202050994"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020104549"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.07.010"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10510"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.02.009"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109146"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45815"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2009.06.002"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.37422"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2014.82"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020506"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2019.00089"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/acn3.51003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008467"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-018-0610-5"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1147554"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4161\/cam.5.2.14403"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.178"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406877102"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260088"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66574"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45559"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.590094"},{"key":"e_1_3_2_39_2","first-page":"1116","article-title":"Genetic analysis of microcephaly-cortical blind syndrome due to compound heterozygous variants of DIAPH1 gene","volume":"39","author":"Li X.","year":"2022","unstructured":"X. Li, P. Lai, K. Cheng, D. Wang, Genetic analysis of microcephaly-cortical blind syndrome due to compound heterozygous variants of DIAPH1 gene. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 39, 1116\u20131119 (2022).","journal-title":"Zhonghua Yi Xue Yi Chuan Xue Za Zhi"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2015.10.003"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506101102"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.12.3367"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.000885"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.29513"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.12.4053-4061.2002"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204715200"},{"key":"e_1_3_2_47_2","first-page":"915","article-title":"The Rho small GTPase: Functions in health and disease","volume":"17","author":"Toksoz D.","year":"2002","unstructured":"D. Toksoz, K. D. Merdek, The Rho small GTPase: Functions in health and disease. Histol. Histopathol. 17, 915\u2013927 (2002).","journal-title":"Histol. Histopathol."},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.11.048"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.157"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2019.00157"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.02-06-0092"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15544"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018019448"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00278-2"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.05.020"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405085101"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.237743.114"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12197"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2017.140"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50354"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171320"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3302"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.200519"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21162"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkae032"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01938"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201711022"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201506110"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232251"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68180-2"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.05.021"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E19-09-0536"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.4.1505"},{"key":"e_1_3_2_74_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90158-2"},{"key":"e_1_3_2_75_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394587-7.00005-1"},{"key":"e_1_3_2_76_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17050707"},{"key":"e_1_3_2_77_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2004427"},{"key":"e_1_3_2_78_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.08.003"},{"key":"e_1_3_2_79_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.4.589"},{"key":"e_1_3_2_80_2","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13259"},{"key":"e_1_3_2_81_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.33273"},{"key":"e_1_3_2_82_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2019.00465"},{"key":"e_1_3_2_83_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000500"},{"key":"e_1_3_2_84_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2016.10.010"},{"key":"e_1_3_2_85_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1213507"},{"key":"e_1_3_2_86_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4807-10.2011"},{"key":"e_1_3_2_87_2","doi-asserted-by":"publisher","DOI":"10.1111\/pde.14923"},{"key":"e_1_3_2_88_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0498-4"},{"key":"e_1_3_2_89_2","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhy073"},{"key":"e_1_3_2_90_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.10.016"},{"key":"e_1_3_2_91_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00772-15"},{"key":"e_1_3_2_92_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.568868"},{"key":"e_1_3_2_93_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2020.118691"},{"key":"e_1_3_2_94_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.4016"},{"key":"e_1_3_2_95_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00432"},{"key":"e_1_3_2_96_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.08.005"},{"key":"e_1_3_2_97_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00362"},{"key":"e_1_3_2_98_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.04.011"},{"key":"e_1_3_2_99_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2015.06.004"},{"key":"e_1_3_2_100_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01906"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.adl4694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T13:59:22Z","timestamp":1721829562000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adl4694"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,26]]},"references-count":99,"journal-issue":{"issue":"30","published-print":{"date-parts":[[2024,7,26]]}},"alternative-id":["10.1126\/sciadv.adl4694"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.adl4694","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.03.11.584337","asserted-by":"object"}]},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,26]]},"assertion":[{"value":"2023-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadl4694"}}