{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:13:59Z","timestamp":1791850439101,"version":"4.3.4"},"reference-count":72,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"22","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,5,31]]},"abstract":"<jats:p>\n            <jats:italic>Streptococcus agalactiae<\/jats:italic>\n            [group B\n            <jats:italic>Streptococcus<\/jats:italic>\n            (GBS)] is a leading cause of neonatal meningitis, with late-onset disease (LOD) occurring after gastrointestinal tract colonization in infants. Bacterial membrane lipids are essential for host-pathogen interactions, and the functions of glycolipids are yet to be fully elucidated. GBS synthesizes three major glycolipids: glucosyl-diacylglycerol (Glc-DAG), diglucosyl-DAG (Glc\n            <jats:sub>2<\/jats:sub>\n            -DAG), and lysyl-Glc-DAG (Lys-Glc-DAG). Here, we identify the enzyme, IagB, as responsible for biosynthesis of Glc-DAG, the precursor for the two other glycolipids in GBS. To examine the collective role of glycolipids to GBS virulence, we adapted a murine model of neonatal meningitis to simulate LOD. The GBS\u2206\n            <jats:italic>iagB<\/jats:italic>\n            mutant traversed the gut-epithelial barrier comparable to wild type but was severely attenuated in bloodstream survival, resulting in decreased bacterial loads in the brain. The GBS\u2206\n            <jats:italic>iagB<\/jats:italic>\n            mutant was more susceptible to neutrophil killing and membrane targeting by host antimicrobial peptides. This work reveals an unexplored function of GBS glycolipids with their ability to protect the bacterial cell from host antimicrobial killing.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adn7848","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T17:58:57Z","timestamp":1717005537000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":20,"title":["<i>Streptococcus agalactiae<\/i>\n            glycolipids promote virulence by thwarting immune cell clearance"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9346-671X","authenticated-orcid":true,"given":"Luke R.","family":"Joyce","sequence":"first","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5037-5068","authenticated-orcid":true,"given":"Sol","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Aurora, CO, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4374-6403","authenticated-orcid":true,"given":"Brady L.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4790-9839","authenticated-orcid":true,"given":"Priya M.","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7343-9271","authenticated-orcid":true,"given":"Kelli L.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8082-3423","authenticated-orcid":true,"given":"Ziqiang","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Duke University Medical Center, Durham, NC, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3700-6441","authenticated-orcid":true,"given":"Julie A.","family":"Siegenthaler","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine, Aurora, CO, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4232-6837","authenticated-orcid":true,"given":"Kelly S.","family":"Doran","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"E. 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