{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:29:58Z","timestamp":1787016598931,"version":"build-2736575974"},"reference-count":35,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"40","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,10,4]]},"abstract":"<jats:p>Tobacco consumption affects human health, but no studies have investigated its effect on the bone metabolome, or if any changes are traceable after long postmortem intervals. Human osteoarchaeological remains preserve small molecules, making them valuable for studies that aim to examine past conditions. We test if there are molecular differences in the metabolome of cortical bone between archaeological individuals who used tobacco and those who did not, and if these differences are distinct enough to assign tobacco use status to individuals with unknown tobacco use. Cortical bone of 323 known and unknown tobacco users was studied by an untargeted metabolomics assay using a liquid chromatography\u2013high-resolution mass spectrometry platform. We identified 45 discriminating molecular features that differed between tobacco consumers (15 up-regulated features) and nonconsumers (17 up-regulated features). Tobacco consumption leaves a metabolic record in human bone distinctive enough to identify its use in individuals of unknown tobacco consumption. Future work will validate molecular features relating to tobacco consumption.<\/jats:p>","DOI":"10.1126\/sciadv.adn9317","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T13:58:42Z","timestamp":1728050322000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":7,"title":["Archaeometabolomics characterizes phenotypic differences in human cortical bone at a molecular level relating to tobacco use"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0588-690X","authenticated-orcid":true,"given":"Diego","family":"Badillo-Sanchez","sequence":"first","affiliation":[{"name":"School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-1468","authenticated-orcid":true,"given":"Anna M.","family":"Davies-Barrett","sequence":"additional","affiliation":[{"name":"School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Serrano","family":"Ruber","sequence":"additional","affiliation":[{"name":"School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6583-870X","authenticated-orcid":true,"given":"Donald J. L.","family":"Jones","sequence":"additional","affiliation":[{"name":"Leicester Cancer Research Centre, RKCSB, University of Leicester, Leicester LE1 7RH, UK."},{"name":"The Leicester van Geest MultiOmics Facility, University of Leicester, Leicester LE1 7RH, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7424-2094","authenticated-orcid":true,"given":"Sarah A.","family":"Inskip","sequence":"additional","affiliation":[{"name":"School of Archaeology and Ancient History, University of Leicester, Leicester LE1 7RH, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr60252a002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41562-021-01202-9"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jas.2005.08.006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jas.2018.10.010"},{"key":"e_1_3_2_6_2","unstructured":"I. Gately Tobacco: A Cultural History of How an Exotic Plant Seduced Civilization (Open Road + Grove\/Atlantic 2007)."},{"key":"e_1_3_2_7_2","unstructured":"J. Hughes Learning to Smoke: Tobacco Use in the West (Univ. of Chicago Press 2003); https:\/\/press.uchicago.edu\/ucp\/books\/book\/chicago\/L\/bo3635395.html."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00008469-200308000-00003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/her\/cyg097"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0018246X21000194"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027001061.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.5.545"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/1354750X.2020.1781260"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19990615)45:4<294::AID-JBM3>3.0.CO;2-1"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003863"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccm.2013.09.011"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4184190"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surge.2009.10.014"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20060173"},{"key":"e_1_3_2_20_2","unstructured":"A. Russell Anthropology of Tobacco: Ethnographic Adventures in Non-Human Worlds (Routledge 2021)."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1177\/096228029800700202"},{"key":"e_1_3_2_22_2","unstructured":"E. Nikita Osteoarchaeology: A Guide to the Macroscopic Study of Human Skeletal Remains (Academic Press 2016)."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-27401-0"},{"key":"e_1_3_2_24_2","first-page":"94","article-title":"Pipe smoking and oral health in males from The Netherlands during the 18th\u201319th century","volume":"57","author":"Inskip S.","year":"2023","unstructured":"S. Inskip, L. Zachary, M. Serrano Ruber, M. Hoogland, Pipe smoking and oral health in males from The Netherlands during the 18th\u201319th century. Postmediev. Archaeol. 57, 94\u2013107 (2023).","journal-title":"Postmediev. Archaeol."},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","unstructured":"Davies-Barrett A. M Inskip S Who smokes anymore? Documentary archaeological and osteological evidence for tobacco consumption and its relationship to social identity in industrial England (AD 1700\u20131850) in The Material Body: Embodiment History and Archaeology in Industrializing England 1700\u20131850 (Manchester Univ. Press 2024).","DOI":"10.7765\/9781526152794.00012"},{"key":"e_1_3_2_26_2","first-page":"8","article-title":"Early career osteology: Developing new specialist skills for the sector","author":"Henderson M.","year":"2020","unstructured":"M. Henderson, Early career osteology: Developing new specialist skills for the sector. The Archaeologist, 8\u20139 (2020).","journal-title":"The Archaeologist"},{"key":"e_1_3_2_27_2","first-page":"17","article-title":"St James\u2019s Burial Ground: Delivering lasting learning","author":"Raynor C.","year":"2020","unstructured":"C. Raynor, St James\u2019s Burial Ground: Delivering lasting learning. The Archaeologist, 17\u201319 (2020).","journal-title":"The Archaeologist"},{"key":"e_1_3_2_28_2","unstructured":"T. Waldron W. Rodwell St Peter\u2019s Barton-upon-Humber Lincolnshire\u2014A Parish Church and Its Community: Volume 2. The Human Remains (Oxbow Books 2007); https:\/\/jstor.org\/stable\/j.ctv2npq989."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/421746"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.4248\/BR201304002"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.161117"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21672"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.15252\/msb.202110240"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116221"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-022-00710-w"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab382"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.adn9317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T11:54:52Z","timestamp":1728647692000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adn9317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,4]]},"references-count":35,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2024,10,4]]}},"alternative-id":["10.1126\/sciadv.adn9317"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.adn9317","relation":{},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,4]]},"assertion":[{"value":"2024-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadn9317"}}