{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:34:55Z","timestamp":1791462895929,"version":"4.3.4"},"reference-count":68,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"39","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,9,27]]},"abstract":"<jats:p>Over the past decades, single-molecule and super-resolution microscopy have advanced and represent essential tools for life science research. There is, however, a growing gap between the state of the art and what is accessible to biologists, biochemists, medical researchers, or labs with financial constraints. To bridge this gap, we introduce Brick-MIC, a versatile and affordable open-source 3D-printed microspectroscopy and imaging platform. Brick-MIC enables the integration of various fluorescence imaging techniques with single-molecule resolution within a single platform and exchange between different modalities within minutes. We here present variants of Brick-MIC that facilitate single-molecule fluorescence detection, fluorescence correlation spectroscopy, time-correlated single-photon counting and super-resolution imaging (STORM and PAINT). Detailed descriptions of the hardware and software components, as well as data analysis routines, are provided, to allow non-optics specialists to operate their own Brick-MIC with minimal effort and investments. We foresee that our affordable, flexible, and open-source Brick-MIC platform will be a valuable tool for many laboratories worldwide.<\/jats:p>","DOI":"10.1126\/sciadv.ado3427","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T15:00:24Z","timestamp":1727276424000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":16,"title":["Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC)"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2797-2005","authenticated-orcid":true,"given":"Gabriel G.","family":"Moya Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Planegg-Martinsried, Germany."},{"name":"Biophysical Chemistry, Faculty of Chemistry and Chemical Biology, Technische Universit\u00e4t Dortmund, Dortmund, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9057-2057","authenticated-orcid":true,"given":"Oliver","family":"Brix","sequence":"additional","affiliation":[{"name":"Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Planegg-Martinsried, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Klocke","sequence":"additional","affiliation":[{"name":"Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Planegg-Martinsried, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5307-3217","authenticated-orcid":true,"given":"Paul D.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Faculty of Mathematics &amp; Science, The Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem, Israel."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3414-8787","authenticated-orcid":true,"given":"Jorge R.","family":"Luna Piedra","sequence":"additional","affiliation":[{"name":"Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Planegg-Martinsried, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5436-3929","authenticated-orcid":true,"given":"Nicolas D.","family":"Wendler","sequence":"additional","affiliation":[{"name":"Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, Planegg-Martinsried, Germany."},{"name":"Biophysical Chemistry, Faculty of Chemistry and Chemical Biology, Technische Universit\u00e4t Dortmund, Dortmund, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-5277","authenticated-orcid":true,"given":"Eitan","family":"Lerner","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Faculty of Mathematics &amp; Science, The Edmond J. 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