{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:39:21Z","timestamp":1791049161785,"version":"4.1.0"},"reference-count":80,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"1","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,1,3]]},"abstract":"<jats:p>\n                    The early detection of neurodegenerative diseases necessitates the identification of specific brain-derived biomolecules in peripheral blood. In this context, our investigation delineates the role of amyloid precursor-like protein 1 (APLP1)\u2014a protein predominantly localized in oligodendrocytes and neurons\u2014as a previously unidentified biomarker in extracellular vesicles (EVs). Through rigorous analysis, APLP1\n                    <jats:sup>+<\/jats:sup>\n                    EVs from human sera were unequivocally determined to be of cerebral origin. This assertion was corroborated by distinctive small RNA expression patterns of APLP1\n                    <jats:sup>+<\/jats:sup>\n                    EVs. The miRNAs\u2019 putative targets within these EVs manifested pronounced expression in the brain, fortifying their neurospecific provenance. We subjected our findings to stringent validation using Thy-1 GFP M line mice, transgenic models wherein GFP expression is confined to hippocampal neurons. An amalgamation of these results with an exhaustive data analysis accentuates the potential of APLP1\n                    <jats:sup>+<\/jats:sup>\n                    EVs as cerebrally originated biomarkers. Synthesizing our findings, APLP1\n                    <jats:sup>+<\/jats:sup>\n                    EVs are postulated not merely as diagnostic markers but as seminal entities shaping the future trajectory of neurodegenerative disease diagnostics.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ado6894","type":"journal-article","created":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T14:03:34Z","timestamp":1735740214000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":14,"title":["Blood-derived APLP1\n                    <sup>+<\/sup>\n                    extracellular vesicles are potential biomarkers for the early diagnosis of brain diseases"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4381-3066","authenticated-orcid":true,"given":"Yuri","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ala","family":"Jo","sequence":"additional","affiliation":[{"name":"Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chul-Woo","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8924-2887","authenticated-orcid":true,"given":"Ji-Min","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Woo","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6587-5623","authenticated-orcid":true,"given":"Kang Soo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, CHA Bundang Medical Center, CHA University College of Medicine, Gyeonggi-do 13496, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7397-5410","authenticated-orcid":true,"given":"Young-Sang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, CHA Bundang Medical Center, CHA University College of Medicine, Gyeonggi-do 13496, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0087-0909","authenticated-orcid":true,"given":"Hakho","family":"Lee","sequence":"additional","affiliation":[{"name":"Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7044-4841","authenticated-orcid":true,"given":"Jisook","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science, CHA University, Gyeonggi-do 13488, Republic of Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","first-page":"289","article-title":"Preclinical progression of neurodegenerative diseases","volume":"80","author":"Katsuno M.","year":"2018","unstructured":"M. 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