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However, the function and mechanism of posttranscriptional chemical modifications, especially the newly identified\n            <jats:italic>N<\/jats:italic>\n            <jats:sup>4<\/jats:sup>\n            -acetylcytidine (ac\n            <jats:sup>4<\/jats:sup>\n            C) modification catalyzed by\n            <jats:italic>N<\/jats:italic>\n            -acetyltransferase 10 (NAT10), are unknown. In this study, we developed a low-input ac\n            <jats:sup>4<\/jats:sup>\n            C sequencing technology, ac\n            <jats:sup>4<\/jats:sup>\n            C LACE-seq, and mapped 8241 ac\n            <jats:sup>4<\/jats:sup>\n            C peaks at the whole-transcriptome level using 50 mouse oocytes at the germinal vesicle stage. Oocyte-specific\n            <jats:italic>Nat10<\/jats:italic>\n            knockout wiped out ac\n            <jats:sup>4<\/jats:sup>\n            C signals in oocytes and caused severe defects in meiotic maturation and female infertility. Mechanically,\n            <jats:italic>Nat10<\/jats:italic>\n            deletion led to a failure of ac\n            <jats:sup>4<\/jats:sup>\n            C deposition on mRNAs encoding key maternal factors, which regulate transcriptome stability and maternal-to-zygotic transition.\n            <jats:italic>Nat10<\/jats:italic>\n            -deleted oocytes showed decreased mRNA translation efficiency due to the direct inhibition of ac\n            <jats:sup>4<\/jats:sup>\n            C sites on specific transcripts during meiotic maturation. In summary, we developed a low-input, high-sensitivity mRNA ac\n            <jats:sup>4<\/jats:sup>\n            C profiling approach and highlighted the important physiological function of ac\n            <jats:sup>4<\/jats:sup>\n            C in the precise regulation of oocyte meiotic maturation by enhancing translation efficiency.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adp5163","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:00:26Z","timestamp":1740164426000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":23,"title":["NAT10-mediated mRNA\n            <i>N<\/i>\n            <sup>4<\/sup>\n            -acetylation is essential for the translational regulation during oocyte meiotic maturation in 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