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Our spatial transcriptomics\u2013based \u201cmechanomics\u201d platform facilitates spatially resolved analysis of the molecular profiles of cells with respect to their local in vivo mechanical environment by integrating time-lapsed in vivo micro\u2013computed tomography, spatial transcriptomics, and micro\u2013finite element analysis. We investigate the transcriptomic responses of cells as a function of the local strain magnitude by identifying the differential expression of genes in regions of high and low strain within a fracture site. 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