{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:54:11Z","timestamp":1791500051316,"version":"4.3.4"},"reference-count":56,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"50","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,12,13]]},"abstract":"<jats:p>\n            Multiple antibiotic resistances are a major global health threat. The predominant tool for adaptation in Gram-negative bacteria is the integron. Under stress, it rearranges gene cassettes to offer an escape using the tyrosine recombinase IntI, recognizing folded DNA hairpins, the\n            <jats:italic>attC<\/jats:italic>\n            sites. Four recombinases and two\n            <jats:italic>attC<\/jats:italic>\n            sites form the synaptic complex. Yet, for unclear reasons, the recombination efficiency varies greatly. Here, we established an optical tweezers force spectroscopy assay to probe the synaptic complex stability and revealed, for seven combinations of\n            <jats:italic>attC<\/jats:italic>\n            sites, significant variability in the mechanical stability. We found a strong correlation between mechanical stability and recombination efficiency of\n            <jats:italic>attC<\/jats:italic>\n            sites in vivo, indicating a regulatory mechanism from the DNA structure to the macromolecular complex stability. Taking into account known forces during DNA metabolism, we propose that the variation of the integron in vivo recombination efficiency is mediated by the synaptic complex stability. We anticipate that further recombination processes are also affected by their corresponding mechanical stability.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adp8756","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T19:00:22Z","timestamp":1734116422000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["The recombination efficiency of the bacterial integron depends on the mechanical stability of the synaptic complex"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4721-0554","authenticated-orcid":true,"given":"Ekaterina","family":"Vorobevskaia","sequence":"first","affiliation":[{"name":"B CUBE, TU Dresden, Tatzberg 41, 01307 Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3774-249X","authenticated-orcid":true,"given":"C\u00e9line","family":"Loot","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR3525, Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, F-75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6482-6002","authenticated-orcid":true,"given":"Didier","family":"Mazel","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR3525, Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, F-75015 Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6209-2364","authenticated-orcid":true,"given":"Michael","family":"Schlierf","sequence":"additional","affiliation":[{"name":"B CUBE, TU Dresden, Tatzberg 41, 01307 Dresden, Germany."},{"name":"Physics of Life, DFG Cluster of Excellence, TU Dresden, 01062 Dresden, Germany."},{"name":"Faculty of Physics, TU Dresden, 01062 Dresden, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153624"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-102209-163504"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5363.605"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02861.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.1995.1.195"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306466101"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00153.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.38"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172914"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000632"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.151"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02296-16"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1258"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04388-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030255"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01467.x"},{"key":"e_1_3_2_18_2","first-page":"MDNA3-0019-2014","article-title":"The integron: Adaptation on demand","volume":"3","author":"Escudero J. 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