{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T06:26:04Z","timestamp":1792823164578,"version":"4.5.0"},"reference-count":57,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"41","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,10,11]]},"abstract":"<jats:p>\n                    Here, we report a magnetogenetic system, based on a single anti-ferritin nanobody-TRPV1 receptor fusion protein, which regulated neuronal activity when exposed to magnetic fields. Adeno-associated virus (AAV)\u2013mediated delivery of a floxed nanobody-TRPV1 into the striatum of adenosine-2a receptor\u2013Cre drivers resulted in motor freezing when placed in a magnetic resonance imaging machine or adjacent to a transcranial magnetic stimulation device. Functional imaging and fiber photometry confirmed activation in response to magnetic fields. Expression of the same construct in the striatum of wild-type mice along with a second injection of an AAVretro expressing Cre into the globus pallidus led to similar circuit specificity and motor responses. Last, a mutation was generated to gate chloride and inhibit neuronal activity. Expression of this variant in the subthalamic nucleus in PitX2-Cre parkinsonian mice resulted in reduced\n                    <jats:italic>c-fos<\/jats:italic>\n                    expression and motor rotational behavior. These data demonstrate that magnetogenetic constructs can bidirectionally regulate activity of specific neuronal circuits noninvasively in vivo using clinically available devices.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adp9150","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T13:58:51Z","timestamp":1728482331000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":20,"title":["Bidirectional regulation of motor circuits using magnetogenetic gene therapy"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7319-6794","authenticated-orcid":true,"given":"Santiago R.","family":"Unda","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8117-7355","authenticated-orcid":true,"given":"Lisa E.","family":"Pomeranz","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7609-7000","authenticated-orcid":true,"given":"Roberta","family":"Marongiu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1712-331X","authenticated-orcid":true,"given":"Xiaofei","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Fudan University, Shanghai 200433, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah","family":"Kelly","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gholamreza","family":"Hassanzadeh","sequence":"additional","affiliation":[{"name":"VIB Nanobody Core, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-4990","authenticated-orcid":true,"given":"Henrik","family":"Molina","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10019, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Vaisey","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Putianqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7170-488X","authenticated-orcid":true,"given":"Jonathan P.","family":"Dyke","sequence":"additional","affiliation":[{"name":"Citigroup Bioimaging Center, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3390-1548","authenticated-orcid":true,"given":"Edward K.","family":"Fung","sequence":"additional","affiliation":[{"name":"Citigroup Bioimaging Center, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3216-0319","authenticated-orcid":true,"given":"Logan","family":"Grosenick","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1744-0785","authenticated-orcid":true,"given":"Rick","family":"Zirkel","sequence":"additional","affiliation":[{"name":"Meining School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0728-3352","authenticated-orcid":true,"given":"Aldana M.","family":"Antoniazzi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofya","family":"Norman","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1639-5401","authenticated-orcid":true,"given":"Conor M.","family":"Liston","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7800-9596","authenticated-orcid":true,"given":"Chris","family":"Schaffer","sequence":"additional","affiliation":[{"name":"Meining School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4342-9416","authenticated-orcid":true,"given":"Nozomi","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Meining School of Biomedical Engineering, Cornell University, Ithaca, NY 14850, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0253-2663","authenticated-orcid":true,"given":"Sarah A.","family":"Stanley","sequence":"additional","affiliation":[{"name":"Diabetes, Obesity and Metabolism Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10019, USA."},{"name":"Nash Family Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10019, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2152-0868","authenticated-orcid":true,"given":"Jeffrey M.","family":"Friedman","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Rockefeller University, New York, NY 10065, USA."},{"name":"Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8979-2459","authenticated-orcid":true,"given":"Michael G.","family":"Kaplitt","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurosurgery, Department of Neurological Surgery, Weill Cornell Medical College, Cornell University, New York, NY 10065, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-020-00426-z"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(08)70291-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1525"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.324"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.27069"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2020.113889"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4265"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1216753"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3730"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature17183"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1261821"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11434-015-0902-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a034322"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09159"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3071-19.2020"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI90132"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06025.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brs.2021.11.010"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091065"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2014.00076"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2018.01.022"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074549"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70039-4"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60982-9"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29750-2"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.90133"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","unstructured":"A. Guillaumin G. Pietro Serra F. Georges \u00c5. Wall\u00e9n-Mackenzie Optogenetic investigation into the role of the subthalamic nucleus in motor control. bioRxiv 2020.07.08.193359 [Preprint] (2020). https:\/\/doi.org\/10.1101\/2020.07.08.193359.","DOI":"10.1101\/2020.07.08.193359"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1094-148"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2018.158"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2020.00041"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/neuros\/nyab147"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41088-2"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","unstructured":"L. E. Pomeranz R. Li X. A. Yu L. Kelly G. Hassanzadeh H. Molina D. Gross M. I. Brier G. Vaisey P. Wang M. Jimenez-Gonzalez A. Garcia-Ocana J. Dordick J. M. Friedman S. A. Stanley Magnetogenetic cell activation using endogenous ferritin. bioRxiv 2023.06.20.545120 [Preprint] (2023). https:\/\/doi.org\/10.1101\/2023.06.20.545120.","DOI":"10.1101\/2023.06.20.545120"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00016.2015"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.103094"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3171"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2018.04.008"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1080\/17588928.2018.1512482"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-021-01162-6"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-1028-8"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1167093"},{"key":"e_1_3_2_43_2","first-page":"1589","article-title":"Subthalamic GAD gene transfer in Parkinson disease patients who are candidates for deep brain stimulation","volume":"12","author":"During M. J.","year":"2001","unstructured":"M. J. During, M. G. Kaplitt, M. B. Stern, D. Eidelberg, Subthalamic GAD gene transfer in Parkinson disease patients who are candidates for deep brain stimulation. Hum. Gene Ther. 12, 1589\u20131591 (2001).","journal-title":"Hum. Gene Ther."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2017.105"},{"key":"e_1_3_2_45_2","first-page":"1380","article-title":"Long-term effect of unilateral subthalamotomy for Parkinson\u2019s disease","volume":"90","author":"Ricardo Y.","year":"2019","unstructured":"Y. Ricardo, N. Pavon, L. Alvarez, E. Casabona, J. Teijeiro, A. Diaz, C. Maragoto, I. Pedroso, I. Garcia-Maeso, J. U. Manez-Miro, R. Martinez-Fernandez, R. Macias, J. A. Obeso, Long-term effect of unilateral subthalamotomy for Parkinson\u2019s disease. J. Neurol. Neurosurg. Psychiatry 90, 1380\u20131381 (2019).","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2016311"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112178"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2019-321140"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.7b00415"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.17210"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-022-00909-y"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45807"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2019.01.003"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70067-1"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.02.070"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00878-8"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-328-8_29"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.119726"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.adp9150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T13:59:35Z","timestamp":1728482375000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp9150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,11]]},"references-count":57,"journal-issue":{"issue":"41","published-print":{"date-parts":[[2024,10,11]]}},"alternative-id":["10.1126\/sciadv.adp9150"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.adp9150","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.07.13.548699","asserted-by":"object"}]},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,11]]},"assertion":[{"value":"2024-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadp9150"}}