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This mutation alters the unique C terminal domain of an essential SC protein, SYP-4, a likely ortholog of the vertebrate SC protein SIX6OS1. We use three-dimensional stochastic optical reconstruction microscopy (3D-STORM) to interrogate the molecular architecture of the SC from wild-type and mutant\n                    <jats:italic>C. elegans<\/jats:italic>\n                    animals. Using a probabilistic mapping approach to analyze super-resolution image data, we detect changes in the organization of the synaptonemal complex in wild-type animals that coincide with crossover designation. We also found that our\n                    <jats:italic>syp-4<\/jats:italic>\n                    mutant perturbs SC architecture. Our findings add to growing evidence that the SC is an active material whose molecular organization contributes to chromosome-wide crossover regulation.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adq9374","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T13:58:39Z","timestamp":1737554319000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":16,"title":["Dynamic molecular architecture of the synaptonemal complex"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1119-8855","authenticated-orcid":true,"given":"Simone","family":"K\u00f6hler","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3200, USA."},{"name":"Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, 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