{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:29:47Z","timestamp":1784593787913,"version":"3.55.0"},"reference-count":49,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"49","license":[{"start":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T00:00:00Z","timestamp":1733443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.science.org\/content\/page\/science-licenses-journal-article-reuse"}],"content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2024,12,6]]},"abstract":"<jats:p>\n            Viral RNA synthesis of mononegaviruses occurs in cytoplasmic membraneless organelles called inclusion bodies (IBs). Here, we report that IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family\n            <jats:italic>Paramyxoviridae<\/jats:italic>\n            , displayed liquid organelle properties formed by liquid-liquid phase separation. Super-resolution microscopic analysis of MuV IBs demonstrated that nucleocapsid and phospho (P)-proteins formed a cage-like structure and that the viral polymerase adopted a reticular pattern and colocalized with viral RNAs. In addition, we characterized host RNAs localized in MuV IBs by a spatial transcriptome analysis, and found that RNAs containing G-quadruplex motif sequences (G4-RNAs) were concentrated. An in vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in P droplets. Together, our data show that MuV generates IBs with a characteristic cage-like structure and host G4-RNAs play an important role in forming MuV IBs.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adr0359","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T18:58:44Z","timestamp":1733511524000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":10,"title":["Structural and molecular properties of mumps virus inclusion bodies"],"prefix":"10.1126","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8613-4717","authenticated-orcid":true,"given":"Hiroshi","family":"Katoh","sequence":"first","affiliation":[{"name":"Department of Microbiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1090-7162","authenticated-orcid":true,"given":"Ryuichi","family":"Kimura","sequence":"additional","affiliation":[{"name":"Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, Japan."},{"name":"Department of Drug Discovery Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1302-5472","authenticated-orcid":true,"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohei","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Hosogi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3271-6807","authenticated-orcid":true,"given":"Yuki","family":"Kitai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3779-0864","authenticated-orcid":true,"given":"Yukiko","family":"Akahori","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiro","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Virology III, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2654-8803","authenticated-orcid":true,"given":"Michiyo","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0615-7676","authenticated-orcid":true,"given":"Hirotaka","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9147-1438","authenticated-orcid":true,"given":"Noriyo","family":"Nagata","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3820-9542","authenticated-orcid":true,"given":"Tadaki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6440-9954","authenticated-orcid":true,"given":"Yasuyuki","family":"Ohkawa","sequence":"additional","affiliation":[{"name":"Division of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-0054, 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