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Lipopolysaccharide (LPS) stimulation of macrophages trained previously with \u03b2-glucan or heme exhibited significantly enhanced procoagulant activity compared to macrophages stimulated with LPS alone, which was mediated by enhanced acid sphingomyelinase\u2013mediated tissue factor decryption. Furthermore, splenic monocytes isolated from \u03b2-glucan\u2013trained mice revealed enhanced procoagulant activity up to 4 weeks after \u03b2-glucan administration compared to monocytes from control mice over the same time period. Moreover, hematopoietic progenitor cells and bone marrow interstitial fluid from \u03b2-glucan\u2013trained mice had enhanced procoagulant activity compared to control mice. Trained immunity and associated metabolic perturbations may therefore represent an opportunity for targeted intervention in immunothrombotic disease development.<\/jats:p>","DOI":"10.1126\/sciadv.ads0105","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T13:59:41Z","timestamp":1741355981000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":12,"title":["Trained immunity causes myeloid cell hypercoagulability"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2987-126X","authenticated-orcid":true,"given":"Aisling M.","family":"Rehill","sequence":"first","affiliation":[{"name":"Irish Centre for Vascular Biology, School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"National Children\u2019s Research Centre, Our Lady\u2019s Children\u2019s Hospital Crumlin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9245-1447","authenticated-orcid":true,"given":"Se\u00e1n","family":"McCluskey","sequence":"additional","affiliation":[{"name":"Irish Centre for Vascular Biology, School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland."},{"name":"National Children\u2019s Research Centre, Our Lady\u2019s Children\u2019s Hospital Crumlin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5546-2910","authenticated-orcid":true,"given":"Anna E.","family":"Ledwith","sequence":"additional","affiliation":[{"name":"School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0278-1878","authenticated-orcid":true,"given":"Tristram A. 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