{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:27:01Z","timestamp":1787016421384,"version":"build-2736575974"},"reference-count":70,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"19","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,5,9]]},"abstract":"<jats:p>\n                    Integrin Mac-1 plays a critical role in the development of multiple sclerosis (MS); however, the underlying mechanism is not fully understood. Here, we developed a myeloid-specific Mac-1\u2013deficient mouse. Using an experimental autoimmune encephalomyelitis (EAE) mouse model of MS, we report that Mac-1 on myeloid cells is key to disease development. Our data reveal that myeloid-specific Mac-1 significantly increases EAE severity and hinders disease regression. Loss of Mac-1 increases Gr-1\n                    <jats:sup>+<\/jats:sup>\n                    cells in peripheral tissues and the CNS and preferably accelerates the transition of Ly6C\n                    <jats:sup>hi<\/jats:sup>\n                    monocytes from a pro-inflammatory to an immunosuppressive phenotype in a disease stage\u2013dependent manner. Mechanistically, our results demonstrate that Mac-1 suppresses interferon-\u03b3 production and prevents monocytes from acquiring immunosuppressive functions by reducing the expression of iNOS, IDO, and CD84. Administration of a NOS-specific inhibitor in Mac-1\u2013deficient EAE mice abolishes disease regression. These insights could help develop Mac-1\u2013targeting strategies for better treatment of MS.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.ads3728","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T13:58:21Z","timestamp":1746799101000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":2,"title":["Mac-1 regulates disease stage\u2013specific immunosuppression via the nitric oxide pathway in autoimmune disease"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0799-8171","authenticated-orcid":true,"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Physiology, Center for Vascular and Inflammatory Diseases, School of Medicine, University of Maryland, Baltimore, Baltimore, MD, 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