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MprF presents a promising target in multiresistant pathogens, but structural information is limited and both substrate specificity and energization of MprF-mediated lipid transport are poorly understood. Here, we present the cryo-EM structure of MprF from\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            (\n            <jats:italic>Pa<\/jats:italic>\n            MprF) bound to a synthetic nanobody.\n            <jats:italic>Pa<\/jats:italic>\n            MprF adopts an \u201copen\u201d conformation with a wide, lipid-exposed groove on the periplasmic side that induces a local membrane deformation in molecular dynamics simulations. Using an in vitro liposome transport assay, we demonstrate that\n            <jats:italic>Pa<\/jats:italic>\n            MprF translocates a wide range of different lipids without an external energy source. This suggests that\n            <jats:italic>Pa<\/jats:italic>\n            MprF is the first dedicated lipid scramblase to be characterized in bacteria.\n          <\/jats:p>","DOI":"10.1126\/sciadv.ads9135","type":"journal-article","created":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T17:58:41Z","timestamp":1744221521000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":12,"title":["MprF from\n            <i>Pseudomonas aeruginosa<\/i>\n            is a promiscuous lipid scramblase with broad substrate specificity"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2655-3247","authenticated-orcid":true,"given":"Matthew T. 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