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The\n            <jats:italic toggle=\"yes\">Drosophila<\/jats:italic>\n            testis-nascent-myotube system allows dissection of contact-dependent migration in vivo at high resolution. Here, we describe a role for the axon guidance factor Plexin A in collective cell migration: maintaining cell-cell interfaces at a precise point on the mesenchymal-to-epithelial continuum. This is crucial for testis myotubes to migrate as a continuous sheet, allowing normal sculpting-morphogenesis. Cells must maintain filopodial N-cadherin\u2013based junctions and remain ECM-tethered near cell-cell contacts to spread while collectively moving. Our data further suggest Semaphorin 1b is a Plexin A antagonist, fine-tuning activation. This reveals a contact-dependent mechanism to maintain sheet integrity during migration, driving organ morphogenesis. This is relevant for mesenchymal organ sculpting in other migratory contexts such as angiogenesis.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adu3741","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T18:00:58Z","timestamp":1750269658000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":5,"title":["Plexin\/Semaphorin antagonism orchestrates collective cell migration and organ sculpting by regulating epithelial-mesenchymal balance"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6995-8159","authenticated-orcid":true,"given":"Maik C.","family":"Bischoff","sequence":"first","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, CB#3280, Chapel Hill, NC 27599-3280, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1017-7323","authenticated-orcid":true,"given":"Jenevieve E.","family":"Norton","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, CB#3280, Chapel Hill, NC 27599-3280, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4478-8202","authenticated-orcid":true,"given":"Sarah E.","family":"Clark","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, CB#3280, Chapel Hill, NC 27599-3280, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1412-3987","authenticated-orcid":true,"given":"Mark","family":"Peifer","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, CB#3280, Chapel Hill, NC 27599-3280, USA."},{"name":"Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2720"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2015.14"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201508047"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.172056"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3438"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02373-8"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nphys3040"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.08.003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20563-9"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.4161\/cam.5.6.18623"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202100124"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20362-2"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"M. 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