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To investigate the absolute configuration of 3,5-dihydroxyhexanoyl (dHh) and to evaluate the structure-activity relationship of the O-antigen trisaccharide repeating unit, we completed total synthesis of four potential trisaccharide isomers, along with 11 additional oligosaccharide fragments of the O-antigen. Stereoselective reduction was used for the synthesis of dHh, and the efficient assembly of dHh and (\n            <jats:italic>R<\/jats:italic>\n            )-3-hydroxybutanoyl (\n            <jats:italic>R<\/jats:italic>\n            Hb) was achieved through a post-glycosylation modification strategy. Through NMR analysis, the absolute configuration of dHh was assigned 3\n            <jats:italic>S<\/jats:italic>\n            ,5\n            <jats:italic>S<\/jats:italic>\n            . Glycan microarray screening indicated that\n            <jats:italic>R<\/jats:italic>\n            Hb is essential for the antigenicity of O-antigen. The nonreducing end disaccharide\n            <jats:bold>59<\/jats:bold>\n            may serve as the minimal antigenic epitope. These findings are an important step toward the design of semi-synthetic carbohydrate vaccines against\n            <jats:italic>V. cholerae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1126\/sciadv.adv0571","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T17:59:57Z","timestamp":1745603997000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["Chemical synthesis elucidates the absolute configuration and key antigenic epitope of\n            <i>Vibrio cholerae<\/i>\n            serotype O100 O-antigen"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3024-4784","authenticated-orcid":true,"given":"Guodong","family":"Chen","sequence":"first","affiliation":[{"name":"School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, P.R. 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