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A double-antibody sandwich approach was used to detect C-peptide, and an antibody regeneration strategy was applied to remove bound antigen-antibody complexes, thereby achieving repeatable detection, enabling at least 10 detection assays on the same chip with negligible signal attenuation. The compact design features a skin contact area of only 3 cm\u00a0by\u00a05.4 cm and excellent biocompatibility. Integrated with a mobile app, the device provides real-time concentration reading. Mouse and rabbit tests confirm effective ISF sampling and monitoring accuracy, demonstrating its potential for at-home diabetes management and broader wearable biosensing applications.<\/jats:p>","DOI":"10.1126\/sciadv.adw2182","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T17:58:34Z","timestamp":1752861514000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":25,"title":["A wearable device for continuous immunoassay-based monitoring of C-peptide in interstitial fluid"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2040-0541","authenticated-orcid":true,"given":"Shengbo","family":"Chen","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Advanced Biomaterials, Shenzhen Key Laboratory of Smart Healthcare Engineering, Department of Biomedical Engineering, Southern University of Science and Technology, No. 1088 Xueyuan Road, 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