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Cryo\u2013electron tomography (cryo-ET) and subtomogram averaging (STA) structural analyses reveal that HTT\u2019s N-terminal HEAT and Bridge domains wrap around F-actin, while the C-terminal HEAT domain is displaced; furthermore, HTT dimerizes via the N-HEAT domain to bridge parallel actin filaments separated by ~20\u00a0nanometers. Our study provides the structural basis for understanding how HTT interacts with and organizes the actin cytoskeleton.<\/jats:p>","DOI":"10.1126\/sciadv.adw4124","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T17:58:44Z","timestamp":1758304724000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":13,"title":["Structure of the Huntingtin F-actin complex reveals its role in cytoskeleton organization"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-7621","authenticated-orcid":true,"given":"R\u00e9mi","family":"Carpentier","sequence":"first","affiliation":[{"name":"Univ. 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