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Here, we report a linear transducer array\u2013based hybrid microscope (LiTA-HM) that integrates photoacoustic microscopy and confocal fluorescence microscopy to achieve simultaneous neurovascular imaging with a field of view (FOV) of 6 millimeters by 5 millimeters, a spatial resolution of ~6 micrometers, and a temporal resolution of 0.8 seconds. We design an array of eight miniature transducers to enlarge FOV and optimize a polygon mirror\u2013based ultrafast scanner to achieve real-time imaging. Proof-of-concept imaging experiments were conducted on awake mice. Different couplings between thousands of neuron somas and vessel branches down to capillaries were observed in hypoxia and anesthesia experiments. Spatiotemporal correlations and functional connectivity in NVC were uncovered for spreading depolarization waves occurred in a drug-induced epilepsy experiment. We expect that LiTA-HM will enable broad applications of neuroimaging.<\/jats:p>","DOI":"10.1126\/sciadv.adw5275","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T17:58:39Z","timestamp":1753293519000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":26,"title":["Photoacoustic and fluorescence hybrid microscope for cortex-wide imaging of neurovascular dynamics with subcellular resolution"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1701-6848","authenticated-orcid":true,"given":"Liangjian","family":"Liu","sequence":"first","affiliation":[{"name":"Research Center for Biomedical Optics and Molecular Imaging, State Key Laboratory of Biomedical Imaging Science and System, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China."},{"name":"University of Chinese Academy of Sciences, No. 19(A) Yuquan Road, Shijingshan 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