{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:22:55Z","timestamp":1780399375549,"version":"3.54.1"},"reference-count":68,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"37","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,9,12]]},"abstract":"<jats:p>\n                    Oxidase-based electrochemical biosensors are widely deployed for point-of-use applications, yet oxygen interference remains a major challenge, substantially reducing sensing accuracy. Here, we developed a universal enzymatic O\n                    <jats:sub>2<\/jats:sub>\n                    scavenger composed of alcohol oxidase, catalase, and paraformaldehyde to eliminate O\n                    <jats:sub>2<\/jats:sub>\n                    within the sensor by converting it to water. Unlike other oxidases, alcohol oxidase exclusively uses O\n                    <jats:sub>2<\/jats:sub>\n                    as an electron acceptor, preventing interference with the electron transfer chain involving the sensing oxidase. We demonstrated the compatibility of this O\n                    <jats:sub>2<\/jats:sub>\n                    scavenger for calibration-free sensing of glucose, lactate, and creatinine in the concentration range relevant to human health. Without the O\n                    <jats:sub>2<\/jats:sub>\n                    scavenger, sensor readings were less than 50% of those under inert gas conditions. With the O\n                    <jats:sub>2<\/jats:sub>\n                    scavenger, the accuracy improved to 99%, even at low substrate concentrations. The general compatibility and performances of this alcohol oxidase-based O\n                    <jats:sub>2<\/jats:sub>\n                    scavenger unlock the full potential of oxidase-based biosensors for reliable point-of-use sensing.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adw6133","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:58:34Z","timestamp":1757699914000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":5,"title":["A universal oxygen scavenger for oxidase-based biosensors"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8149-8637","authenticated-orcid":true,"given":"Huijie","family":"Zhang","sequence":"first","affiliation":[{"name":"School of New Energy, Nanjing University of Science and Technology, Jiangyin, Jiangsu 214443, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed G.","family":"Saadeldin","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6775-768X","authenticated-orcid":true,"given":"Darren","family":"Buesen","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamzah","family":"Elfaitory","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Process Engineering, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9376-9438","authenticated-orcid":true,"given":"Jakob","family":"Burger","sequence":"additional","affiliation":[{"name":"Laboratory of Chemical Process Engineering, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4277-8473","authenticated-orcid":true,"given":"Vincent M.","family":"Friebe","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7922-8866","authenticated-orcid":true,"given":"Jonas","family":"Honacker","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0844-2375","authenticated-orcid":true,"given":"Tobias","family":"V\u00f6pel","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6056-0507","authenticated-orcid":true,"given":"Alaa A.","family":"Oughli","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5303-7865","authenticated-orcid":true,"given":"Nicolas","family":"Plumer\u00e9","sequence":"additional","affiliation":[{"name":"Professorship for Electrobiotechnology, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Uferstra\u00dfe 53, 94315 Straubing, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00759"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2022.340517"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45789-9"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS00304B"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adl1856"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi3839"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abd0199"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.64035"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr068069y"},{"key":"e_1_3_2_11_2","unstructured":"R. N. Pittman \u201cOxygen transport\u201d in Regulation of Tissue Oxygenation (Morgan & Claypool Life Sciences 2011)."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac2020883"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-013-6827-z"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2015.04.057"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114720"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/bios11040098"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1039\/b212917e"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2006.08.041"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2012.02.053"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1149\/2.1671704jes"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelechem.2004.08.014"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2018.06.047"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie00061a001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0039-9140(94)80110-X"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-0728(73)80037-3"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/s8128237"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0039-9140(67)80124-3"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2013.12.127"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.118.3068.447"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90454-4"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/nn301895c"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.23.55"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:MCBI.0000026056.75937.98"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00663998"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.09.013"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(81)90212-5"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.3109\/10520290009047980"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.iecr.1c04275"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1177\/193229681100500507"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr068123a"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2013.08.173"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1089\/dia.2013.0184"},{"key":"e_1_3_2_43_2","first-page":"149","article-title":"Continuous glucose monitoring in hypoxic environments based on water splitting-assisted electrocatalysis","volume":"11","author":"Lei L.","year":"2023","unstructured":"L. Lei, C. Xu, X. Dong, B. Ma, Y. Chen, Q. Hao, C. Zhao, H. Liu, Continuous glucose monitoring in hypoxic environments based on water splitting-assisted electrocatalysis. Chem 11, 149 (2023).","journal-title":"Chem"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1021\/ma00222a008"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/met9040387"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00071a031"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-703X(02)00120-1"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00865a018"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0956-5663(94)80016-2"},{"key":"e_1_3_2_50_2","unstructured":"T. K. Mathew M. Zubair P. Tadi \u201cBlood glucose monitoring\u201d in StatPearls (StatPearls Publishing 2024)."},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(80)90093-1"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4005(01)00624-4"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2021.338748"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2018.06.054"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2017.12.018"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matdes.2024.112746"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1039\/C4CC01670J"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.electacta.2013.09.018"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/celc.201600357"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/elan.201700785"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113054"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-019-1108-y"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2018.11.031"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.12.018"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1039\/C9SC03653A"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0104(96)00253-4"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1002\/aic.690100218"},{"key":"e_1_3_2_68_2","first-page":"2279","article-title":"Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase","volume":"44","author":"Couderc R.","year":"1980","unstructured":"R. Couderc, J. Baratti, Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the alcohol oxidase. Agric. Biol. Chem. 44, 2279\u20132289 (1980).","journal-title":"Agric. Biol. Chem."},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1021\/je950222q"}],"container-title":["Science Advances"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.science.org\/doi\/pdf\/10.1126\/sciadv.adw6133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T17:59:41Z","timestamp":1757699981000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adw6133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,12]]},"references-count":68,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2025,9,12]]}},"alternative-id":["10.1126\/sciadv.adw6133"],"URL":"https:\/\/doi.org\/10.1126\/sciadv.adw6133","relation":{"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv-2024-trlwf","asserted-by":"object"},{"id-type":"doi","id":"10.26434\/chemrxiv-2024-trlwf-v2","asserted-by":"object"}]},"ISSN":["2375-2548"],"issn-type":[{"value":"2375-2548","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,12]]},"assertion":[{"value":"2025-02-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"eadw6133"}}