{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:12:14Z","timestamp":1788405134560,"version":"build-2803163510"},"reference-count":92,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"36","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,9,5]]},"abstract":"<jats:p>\n                    Aminoglycosides (AGs) are broad-spectrum antibiotics effective against Gram-negative bacteria, with uptake dependent on membrane potential. However, the mechanisms of AG entry remain incompletely understood. Here, we identify a previously undescribed uptake pathway via carbohydrate transporters in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    . By deleting or overexpressing 26 carbohydrate transporters, we found that 18 facilitated AG uptake, a mechanism conserved across several Gram-negative ESKAPEE pathogens. Using fluorescent-labeled AGs and flow cytometry, we quantified differential uptake. To enhance AG efficacy, we screened 198 carbon sources for their ability to induce transporter expression using a\n                    <jats:italic toggle=\"yes\">cmtA<\/jats:italic>\n                    -\n                    <jats:italic toggle=\"yes\">gfp<\/jats:italic>\n                    fusion. Uridine emerged as a strong inducer of\n                    <jats:italic toggle=\"yes\">cmtA<\/jats:italic>\n                    and 12 additional AG-importing transporters. Coadministration of uridine considerably improved AG efficacy against clinical and resistant\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    strains by enhancing drug uptake. This combination also improved outcomes in human blood ex vivo and in a murine urinary tract infection model. Given uridine\u2019s clinical safety, it holds promise as an adjuvant to potentiate AG treatment against multidrug-resistant infections.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adw7630","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T17:58:29Z","timestamp":1757095109000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":2,"title":["Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7843-4747","authenticated-orcid":true,"given":"Manon","family":"Lang","sequence":"first","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR3525, Unit\u00e9 Plasticit\u00e9 du G\u00e9nome Bact\u00e9rien, 75015 Paris, France."},{"name":"Sorbonne Universit\u00e9, Coll\u00e8ge Doctoral, F-75005 Paris, France."},{"name":"Evotec ID (Lyon) SAS, 69000 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