{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:15:41Z","timestamp":1791504941323,"version":"4.3.4"},"reference-count":71,"publisher":"American Association for the Advancement of Science (AAAS)","issue":"28","content-domain":{"domain":["www.science.org"],"crossmark-restriction":true},"short-container-title":["Sci. Adv."],"published-print":{"date-parts":[[2025,7,11]]},"abstract":"<jats:p>\n            Powassan virus (POWV) is an emerging tick-borne flavivirus for which no vaccine or antiviral treatment exists. The incidence of human infections of POWV in North America has been increasing because of the expanding distribution of the tick vector\n            <jats:italic>Ixodes scapularis<\/jats:italic>\n            that transmits POWV to humans. To mitigate the dangers of handling a risk group 3 human pathogen, a chimeric virus was constructed from the genetic backbone of a yellow fever virus vaccine strain 17D (YFV-17D) and the external structural proteins of POWV lineage II. The chimera had a comparable phenotype as POWV. The atomic resolution of yellow fever virus-Powassan virus chimera (yPOWV) structure was built without ambiguity, revealing surface glycans and lipid pocket factors. The similarity to other flavivirus structures and the phenotype similar to YFV-17D suggest that there could be future potential as a vaccine candidate.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adw7700","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T17:58:57Z","timestamp":1752083937000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":4,"title":["Atomic-resolution structure of a chimeric Powassan tick-borne flavivirus"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8925-6832","authenticated-orcid":true,"given":"Sayan","family":"Das","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6589-5285","authenticated-orcid":true,"given":"Anoop","family":"Narayanan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim M.","family":"Moustafa","sequence":"additional","affiliation":[{"name":"The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8191-7181","authenticated-orcid":true,"given":"Sung Hyun","family":"Cho","sequence":"additional","affiliation":[{"name":"The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3171-8093","authenticated-orcid":true,"given":"Dana","family":"Mitzel","sequence":"additional","affiliation":[{"name":"National Bio- and Agro-Defense Facility, Agricultural Research Service, US Department of Agriculture, Manhattan, KS, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1261-6921","authenticated-orcid":true,"given":"Joyce","family":"Jose","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA."},{"name":"The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2609-4036","authenticated-orcid":true,"given":"Susan L.","family":"Hafenstein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA."},{"name":"Institute for Molecular Virology, University of Minnesota, Minneapolis, MN, USA."},{"name":"Department of Infectious Diseases, Mayo Clinic, Rochester, MN, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-023-05835-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085446"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2017.2110"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2020.101540"},{"key":"e_1_3_2_6_2","first-page":"708","article-title":"Powassan virus: Isolation of virus from a fatal case of encephalitis","volume":"80","author":"McLEAN D. 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