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We characterized the structure of b-Cool by LC-MS and NMR and found that it is prevalent across a diverse range of bordetellae, including\n            <jats:italic toggle=\"yes\">Bordetella pertussis<\/jats:italic>\n            , which causes whooping cough in humans. A\n            <jats:italic toggle=\"yes\">B. bronchiseptica<\/jats:italic>\n            mutant lacking b-Cool (\u0394\n            <jats:italic toggle=\"yes\">b-Cool<\/jats:italic>\n            ) showed significantly delayed and decreased colonization in mouse nasopharynx and nasal epithelia, resulting in decreased transmission. The colonization defect of \u0394\n            <jats:italic toggle=\"yes\">b-Cool<\/jats:italic>\n            was rescued in mucin deficient mice, suggesting that b-Cool may facilitate colonization in the presence of airway mucins.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adw7764","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T17:58:46Z","timestamp":1756922326000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":0,"title":["Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7720-4197","authenticated-orcid":true,"given":"Yang","family":"Su","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA."},{"name":"Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 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