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Here, we combine recombinase polymerase amplification with Cas13a and Cas12a detection to create two parallelized one-pot assays that detect two conserved elements of\n            <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n            (\n            <jats:italic toggle=\"yes\">IS6110<\/jats:italic>\n            and\n            <jats:italic toggle=\"yes\">IS1081<\/jats:italic>\n            ) and a human DNA internal control. These assays are compatible with lateral flow and can be readily lyophilized. Our final assay showed a limit of detection of 69.0 CFU per milliliter for\n            <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n            H37Rv and 80.5 CFU per milliliter for\n            <jats:italic toggle=\"yes\">Mycobacterium bovis<\/jats:italic>\n            BCG in spiked sputum, with no cross-reactivity to diverse bacterial or fungal isolates. Clinical tests on 13 blinded sputum samples revealed 100% (six of six) sensitivity and 100% (seven of seven) specificity compared to culture. SHINE-TB streamlines TB diagnosis from sample to answer by combining amplification and detection while being compatible with lateral flow and lyophilization.\n          <\/jats:p>","DOI":"10.1126\/sciadv.adx2067","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T17:58:27Z","timestamp":1754503107000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":44,"title":["A streamlined CRISPR-based test for tuberculosis detection directly from sputum"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2036-0455","authenticated-orcid":true,"given":"Alexandra G.","family":"Bell","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton NJ 08544, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6495-3657","authenticated-orcid":true,"given":"Owen R. 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