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Here, we demonstrate that this effect is accompanied by reduction of cerebral edema, attenuated astrocyte dysfunction, and a shift in serum protein composition toward an anti-inflammatory, neuroprotective state. DMT restored tight junction integrity and blood-brain barrier (BBB) function in vitro and in vivo. DMT suppressed the release of proinflammatory cytokines and chemokines in brain endothelial cells and peripheral immune cells and reduced microglial activation via the sigma-1 receptor. Our findings prove that DMT mitigates a poststroke effect by stabilizing the BBB and reducing neuroinflammation. Such interactions of DMT with the vascular and immune systems can be leveraged to complement current, insufficient, stroke therapy.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adx5958","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T17:59:04Z","timestamp":1755107944000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":19,"title":["<i>N<\/i>\n                    ,\n                    <i>N<\/i>\n                    -dimethyltryptamine mitigates experimental stroke by stabilizing the blood-brain barrier and reducing neuroinflammation"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5025-9932","authenticated-orcid":true,"given":"Marcell J.","family":"L\u00e1szl\u00f3","sequence":"first","affiliation":[{"name":"Heart and Vascular Centre, Semmelweis University, Budapest, 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