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To address this, we present Stressomic, a wearable multiplexed microfluidic biosensor for noninvasive monitoring of cortisol, epinephrine, and norepinephrine in sweat. Stressomic integrates iontophoresis-driven sweat extraction with bursting valve-regulated microfluidic channels for continuous sampling and analysis. Gold nanodendrite\u2013decorated laser-engraved graphene electrodes achieve picomolar-level sensitivity, enabling simultaneous detection of multiple stress hormones. Electrochemical assays and human studies demonstrate that Stressomic reliably tracks hormone fluctuations in response to physical, psychological, and pharmacological stressors. Distinct temporal patterns reveal the dynamic interplay between the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system. This platform enables continuous, multiplexed stress profiling, offering opportunities for early detection of maladaptive responses, personalized stress management, and deeper insights into stress biology.<\/jats:p>","DOI":"10.1126\/sciadv.adx6491","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T17:58:27Z","timestamp":1754503107000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":99,"title":["Stressomic: A wearable microfluidic biosensor for dynamic profiling of multiple stress hormones in sweat"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7653-6640","authenticated-orcid":true,"given":"Jiaobing","family":"Tu","sequence":"first","affiliation":[{"name":"Andrew and Peggy Cherng Department of Medical Engineering, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA, 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