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Adv."],"published-print":{"date-parts":[[2025,12,12]]},"abstract":"<jats:p>\n                    Redox imbalance is a key factor in the pathogenesis of diseases such as epilepsy and liver injury. Superoxide anion (O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    ), cysteine (Cys), and homocysteine (Hcy) play central roles in maintaining redox homeostasis, and their dysregulation drives oxidative stress and disease progression. Here, we report a multifunctional fluorescent probe, BPC, capable of simultaneously and selectively detecting Cys, Hcy, and O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    in complex biological environments. BPC shows high sensitivity, selectivity, and biocompatibility, enabling real-time visualization of redox fluctuations in living cells and zebrafish with minimal cytotoxicity. In pentylenetetrazole (PTZ)\u2013 and acetaminophen (APAP)\u2013induced models of epilepsy and liver injury, BPC revealed notable alterations in Cys, Hcy, and O\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>\u2022\u2212<\/jats:sup>\n                    levels, providing mechanistic insights into redox dysregulation. Moreover, BPC successfully tracked redox restoration following\n                    <jats:italic toggle=\"yes\">N<\/jats:italic>\n                    -acetylcysteine (NAC) treatment. These findings establish BPC as a versatile tool for redox biology and highlight its promise for diagnostic and therapeutic applications.\n                  <\/jats:p>","DOI":"10.1126\/sciadv.adx6659","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T18:58:57Z","timestamp":1765565937000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":9,"title":["A fluorescent probe for concurrent detection of cysteine, homocysteine, and superoxide anion"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5576-490X","authenticated-orcid":true,"given":"Yang","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Interdisciplinary Studies, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China."},{"name":"Department of Gastroenterology and Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University\/Hunan Cancer Hospital, Changsha 410013, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7113-1886","authenticated-orcid":true,"given":"Ting","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Studies, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2939-5674","authenticated-orcid":true,"given":"Zhaoxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Studies, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China."},{"name":"Department of Gastroenterology and Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University\/Hunan Cancer Hospital, Changsha 410013, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialing","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Studies, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China."},{"name":"Department of Gastroenterology and Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University\/Hunan Cancer Hospital, Changsha 410013, 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Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3534-1469","authenticated-orcid":true,"given":"Huijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University\/Hunan Cancer Hospital, Changsha 410013, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9809-2366","authenticated-orcid":true,"given":"Peng","family":"Yin","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Studies, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, 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