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Next, we solved a 4.2-\u00c5 resolution cryo\u2013electron microscopy (cryo-EM) structure of this 11-nanometer LPL filament using purified protein. The filament is made of repeating pairs of LPL molecules with occluded active sites, rendering the LPL inactive. The comparison of the in situ subtomogram average and the in vitro cryo-EM structure indicates that the previously uncharacterized physiological storage form of LPL is an inactive filament.<\/jats:p>","DOI":"10.1126\/sciadv.adx8711","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T17:58:27Z","timestamp":1754503107000},"update-policy":"https:\/\/doi.org\/10.34133\/aaas_crossmark","source":"Crossref","is-referenced-by-count":1,"title":["Cryogenic electron tomography reveals helical organization of lipoprotein lipase in storage vesicles"],"prefix":"10.1126","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2078-6947","authenticated-orcid":true,"given":"Kathryn H.","family":"Gunn","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11790, USA."},{"name":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2549-8621","authenticated-orcid":true,"given":"Anna","family":"Wheless","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5716-0270","authenticated-orcid":true,"given":"Thomas","family":"Calcraft","sequence":"additional","affiliation":[{"name":"Structural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Kreutzberger","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7825-5447","authenticated-orcid":true,"given":"Kareem","family":"El-Houshy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4844-5212","authenticated-orcid":true,"given":"Edward H.","family":"Egelman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0387-2862","authenticated-orcid":true,"given":"Peter B.","family":"Rosenthal","sequence":"additional","affiliation":[{"name":"Structural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4165-6674","authenticated-orcid":true,"given":"Saskia B.","family":"Neher","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"221","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2023.102530"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-019-00602-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay5359"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2013.03.011"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1916555117"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(76)90194-6"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258734"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)42617-3"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)51615-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1042\/ETLS20240006"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2019.08.014"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0580-y"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2016.07.011"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18952-1"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33957-8"},{"key":"e_1_3_2_17_2","unstructured":"(Comet Technologies Canada Inc. 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